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| Main Authors: | , , , , , , |
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| Format: | Artículo científico |
| Language: | en |
| Published: |
Neuropeptides
2026
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| Online Access: | https://pubmed.ncbi.nlm.nih.gov/42229239/ |
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| _version_ | 1868266041736429569 |
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| author | Pieroni, Eleonora Maria Dillon, James O'Connor, Vincent Holden-Dye, Lindy Imperadore, Pamela Fiorito, Graziano Yañez-Guerra, Luis Alfonso |
| author_facet | Pieroni, Eleonora Maria Dillon, James O'Connor, Vincent Holden-Dye, Lindy Imperadore, Pamela Fiorito, Graziano Yañez-Guerra, Luis Alfonso Pieroni, Eleonora Maria Dillon, James O'Connor, Vincent Holden-Dye, Lindy Imperadore, Pamela Fiorito, Graziano Yañez-Guerra, Luis Alfonso |
| collection | PubMed - marine biology |
| contents | In silico identification and deorphanisation of an allatostatin C GPCR system in the cephalopod Octopus vulgaris reveal two receptors with distinct potency. Pieroni, Eleonora Maria Dillon, James O'Connor, Vincent Holden-Dye, Lindy Imperadore, Pamela Fiorito, Graziano Yañez-Guerra, Luis Alfonso The investigation of the functional roles of peptide signalling represents an important route to understanding evolution of specific physiological traits and behaviours in metazoans. Allatostatins and their cognate receptors are classically defined as invertebrate neuropeptide hormones. Accumulating evidence recognises allatostatin C as a conserved signalling molecule across all invertebrate lineages, with reported functions spanning from regulation of feeding and digestion to immune responses and modulation of core nociception. Their orthologues across phyla imply biological functions of wider evolutionary significance. In particular, the presumed relationship with the somatostatin/opioid signalling receptors, modulating pain in mammals, warrants consideration. Here we combined in silico and experimental approaches to describe the molecular determinants of the allatostatin C signalling in the cephalopod Octopus vulgaris. This organism has been pivotal in understanding complex neurobiology due to the evolution of a large centralised nervous system. Our investigation resolved a single prepropeptide encompassing allatostatin C peptide (OvAstC) and two distinct allatostatin C receptors (OvAstCR1 and OvAstCR2). The single predicted mature peptide (AVITACYFQAVSCY) was shown to differentially activate the two identified cognate receptors. PCR analysis carried out in O. vulgaris tissues showed a broad distribution of OvAstC and OvAstCRs. This wide expression, while including nervous tissues, clearly extends to the immune and digestive systems. This distribution is consistent with a pleiotropic role of this peptidergic system. The neuro-sensory expression and the reinforced opioid/somatostatin-related phylogenetic placement of OvAstC/OvAstCRs, encourage further physiological investigation of the neuromodulatory control of sensory processing, including nociception in cephalopods. |
| format | Artículo científico |
| id | pubmed_42229239 |
| institution | PubMed |
| language | en |
| publishDate | 2026 |
| publisher | Neuropeptides |
| record_format | pubmed |
| spellingShingle | In silico identification and deorphanisation of an allatostatin C GPCR system in the cephalopod Octopus vulgaris reveal two receptors with distinct potency. Pieroni, Eleonora Maria Dillon, James O'Connor, Vincent Holden-Dye, Lindy Imperadore, Pamela Fiorito, Graziano Yañez-Guerra, Luis Alfonso In silico identification and deorphanisation of an allatostatin C GPCR system in the cephalopod Octopus vulgaris reveal two receptors with distinct potency. Pieroni, Eleonora Maria Dillon, James O'Connor, Vincent Holden-Dye, Lindy Imperadore, Pamela Fiorito, Graziano Yañez-Guerra, Luis Alfonso The investigation of the functional roles of peptide signalling represents an important route to understanding evolution of specific physiological traits and behaviours in metazoans. Allatostatins and their cognate receptors are classically defined as invertebrate neuropeptide hormones. Accumulating evidence recognises allatostatin C as a conserved signalling molecule across all invertebrate lineages, with reported functions spanning from regulation of feeding and digestion to immune responses and modulation of core nociception. Their orthologues across phyla imply biological functions of wider evolutionary significance. In particular, the presumed relationship with the somatostatin/opioid signalling receptors, modulating pain in mammals, warrants consideration. Here we combined in silico and experimental approaches to describe the molecular determinants of the allatostatin C signalling in the cephalopod Octopus vulgaris. This organism has been pivotal in understanding complex neurobiology due to the evolution of a large centralised nervous system. Our investigation resolved a single prepropeptide encompassing allatostatin C peptide (OvAstC) and two distinct allatostatin C receptors (OvAstCR1 and OvAstCR2). The single predicted mature peptide (AVITACYFQAVSCY) was shown to differentially activate the two identified cognate receptors. PCR analysis carried out in O. vulgaris tissues showed a broad distribution of OvAstC and OvAstCRs. This wide expression, while including nervous tissues, clearly extends to the immune and digestive systems. This distribution is consistent with a pleiotropic role of this peptidergic system. The neuro-sensory expression and the reinforced opioid/somatostatin-related phylogenetic placement of OvAstC/OvAstCRs, encourage further physiological investigation of the neuromodulatory control of sensory processing, including nociception in cephalopods. |
| title | In silico identification and deorphanisation of an allatostatin C GPCR system in the cephalopod Octopus vulgaris reveal two receptors with distinct potency. |
| url | https://pubmed.ncbi.nlm.nih.gov/42229239/ |