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| Main Authors: | , , , , , , |
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| Format: | Artículo científico |
| Language: | en |
| Published: |
Small (Weinheim an der Bergstrasse, Germany)
2026
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| Online Access: | https://pubmed.ncbi.nlm.nih.gov/42240039/ |
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| _version_ | 1868266041720700929 |
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| author | Lu, Linfang Jiang, Kang Li, Na Li, Xiaoli Madushika, Lakshani Ling, Sumei Wang, Shihua |
| author_facet | Lu, Linfang Jiang, Kang Li, Na Li, Xiaoli Madushika, Lakshani Ling, Sumei Wang, Shihua Lu, Linfang Jiang, Kang Li, Na Li, Xiaoli Madushika, Lakshani Ling, Sumei Wang, Shihua |
| collection | PubMed - marine biology |
| contents | Engineering a High-Affinity Monoclonal Antibody for Thiram Immunoassays and Protection. Lu, Linfang Jiang, Kang Li, Na Li, Xiaoli Madushika, Lakshani Ling, Sumei Wang, Shihua Thiram (TMTD), a widely used dithiocarbamate fungicide, provokes oxidative hepatotoxicity, yet practical strategies for its detection and immunoprotection remain scarce. Here we develop an antibody-enabled framework integrating detection, therapy, and prevention against TMTD. In the murine model, we characterized the dose-dependent hepatic injury induced by TMTD. Guided by rational C/C hapten design, we then generate a monoclonal antibody with high affinity. Building on this reagent, we create a portable immunodetection platform that couples Au nanoflowers with an Au/Ir@Zn/Cu-MOF probe, achieving an IC of 0.246 µg/mL. To evaluate protective efficacy, we demonstrated that passive immunization conferred a clear time-dependent therapeutic benefit: mortality decreased from 37.5% in the TMTD-poisoned group to 0% with treatment at 15 or 30 min and to 12.5% with treatment at 60 min, while improving body weight, liver function, and oxidative-stress markers. Complementarily, active immunization with TMTD-BSA elicited high antibody titers and conferred complete survival with graded histopathological protection. Together, this work offers an integrated detection-to-protection strategy that effectively bridges immunodetection with immune protection against TMTD-induced toxicity. |
| format | Artículo científico |
| id | pubmed_42240039 |
| institution | PubMed |
| language | en |
| publishDate | 2026 |
| publisher | Small (Weinheim an der Bergstrasse, Germany) |
| record_format | pubmed |
| spellingShingle | Engineering a High-Affinity Monoclonal Antibody for Thiram Immunoassays and Protection. Lu, Linfang Jiang, Kang Li, Na Li, Xiaoli Madushika, Lakshani Ling, Sumei Wang, Shihua Engineering a High-Affinity Monoclonal Antibody for Thiram Immunoassays and Protection. Lu, Linfang Jiang, Kang Li, Na Li, Xiaoli Madushika, Lakshani Ling, Sumei Wang, Shihua Thiram (TMTD), a widely used dithiocarbamate fungicide, provokes oxidative hepatotoxicity, yet practical strategies for its detection and immunoprotection remain scarce. Here we develop an antibody-enabled framework integrating detection, therapy, and prevention against TMTD. In the murine model, we characterized the dose-dependent hepatic injury induced by TMTD. Guided by rational C/C hapten design, we then generate a monoclonal antibody with high affinity. Building on this reagent, we create a portable immunodetection platform that couples Au nanoflowers with an Au/Ir@Zn/Cu-MOF probe, achieving an IC of 0.246 µg/mL. To evaluate protective efficacy, we demonstrated that passive immunization conferred a clear time-dependent therapeutic benefit: mortality decreased from 37.5% in the TMTD-poisoned group to 0% with treatment at 15 or 30 min and to 12.5% with treatment at 60 min, while improving body weight, liver function, and oxidative-stress markers. Complementarily, active immunization with TMTD-BSA elicited high antibody titers and conferred complete survival with graded histopathological protection. Together, this work offers an integrated detection-to-protection strategy that effectively bridges immunodetection with immune protection against TMTD-induced toxicity. |
| title | Engineering a High-Affinity Monoclonal Antibody for Thiram Immunoassays and Protection. |
| url | https://pubmed.ncbi.nlm.nih.gov/42240039/ |