Salvato in:
| Autori principali: | , , , , , , |
|---|---|
| Natura: | Artículo científico |
| Lingua: | en |
| Pubblicazione: |
Small (Weinheim an der Bergstrasse, Germany)
2026
|
| Accesso online: | https://pubmed.ncbi.nlm.nih.gov/42240039/ |
| Tags: |
Aggiungi Tag
Nessun Tag, puoi essere il primo ad aggiungerne!!
|
Sommario:
- Engineering a High-Affinity Monoclonal Antibody for Thiram Immunoassays and Protection. Lu, Linfang Jiang, Kang Li, Na Li, Xiaoli Madushika, Lakshani Ling, Sumei Wang, Shihua Thiram (TMTD), a widely used dithiocarbamate fungicide, provokes oxidative hepatotoxicity, yet practical strategies for its detection and immunoprotection remain scarce. Here we develop an antibody-enabled framework integrating detection, therapy, and prevention against TMTD. In the murine model, we characterized the dose-dependent hepatic injury induced by TMTD. Guided by rational C/C hapten design, we then generate a monoclonal antibody with high affinity. Building on this reagent, we create a portable immunodetection platform that couples Au nanoflowers with an Au/Ir@Zn/Cu-MOF probe, achieving an IC of 0.246 µg/mL. To evaluate protective efficacy, we demonstrated that passive immunization conferred a clear time-dependent therapeutic benefit: mortality decreased from 37.5% in the TMTD-poisoned group to 0% with treatment at 15 or 30 min and to 12.5% with treatment at 60 min, while improving body weight, liver function, and oxidative-stress markers. Complementarily, active immunization with TMTD-BSA elicited high antibody titers and conferred complete survival with graded histopathological protection. Together, this work offers an integrated detection-to-protection strategy that effectively bridges immunodetection with immune protection against TMTD-induced toxicity.