Efficient DNA Extraction Coupled with Fluorescence Loop-Mediated Isothermal Amplification (LAMP) for On-Site Early Warning Detection of Vibrio parahaemolyticus.
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| Autori principali: | , , , , , , , , , , |
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| Natura: | Artículo científico |
| Lingua: | en |
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Marine biotechnology (New York, N.Y.)
2026
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| _version_ | 1868266041717555200 |
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| author | Liu, Fengyi Geng, Qilin Sun, Xue Lin, Jie Zhu, Xuan Wang, Danyang Zhang, Xiao-Hua Hu, Jingjie Wang, Shi Bao, Lisui Lv, Jia |
| author_facet | Liu, Fengyi Geng, Qilin Sun, Xue Lin, Jie Zhu, Xuan Wang, Danyang Zhang, Xiao-Hua Hu, Jingjie Wang, Shi Bao, Lisui Lv, Jia Liu, Fengyi Geng, Qilin Sun, Xue Lin, Jie Zhu, Xuan Wang, Danyang Zhang, Xiao-Hua Hu, Jingjie Wang, Shi Bao, Lisui Lv, Jia |
| collection | PubMed - marine biology |
| contents | Efficient DNA Extraction Coupled with Fluorescence Loop-Mediated Isothermal Amplification (LAMP) for On-Site Early Warning Detection of Vibrio parahaemolyticus. Liu, Fengyi Geng, Qilin Sun, Xue Lin, Jie Zhu, Xuan Wang, Danyang Zhang, Xiao-Hua Hu, Jingjie Wang, Shi Bao, Lisui Lv, Jia Vibrio parahaemolyticus Nucleic Acid Amplification Techniques DNA, Bacterial Animals Sensitivity and Specificity Aquaculture Fluorescence Seawater Molecular Diagnostic Techniques Vibrio Infections Vibrio parahaemolyticus (V. parahaemolyticus), a significant pathogen in aquatic products, necessitates rapid and precise detection to prevent foodborne disease outbreaks. Early detection of bacterial pathogens is crucial for effective prevention and control. In this study, we developed a field-deployable, "sample-in, answer-out" workflow that integrates efficient DNA extraction with visual fluorescence loop-mediated isothermal amplification (LAMP) for the on-site detection of V. parahaemolyticus. This extraction protocol enables efficient one-pot DNA release within 10 min, eliminating the need for additional purification steps. In addition, coupled with an optimized fluorescence LAMP assay targeting V. parahaemolyticus, the entire process-from sample to result-can be completed within approximately 50 min. The optimized assay exhibited high sensitivity, detecting as low as 1 copy/µL of plasmid DNA and 10 CFU per reaction from pure bacterial culture and spiked seawater samples. This extraction-amplification integrated approach demonstrates robust performance and high practicality for on-site early warning and pathogen monitoring in aquaculture safety settings. |
| format | Artículo científico |
| id | pubmed_42240781 |
| institution | PubMed |
| language | en |
| publishDate | 2026 |
| publisher | Marine biotechnology (New York, N.Y.) |
| record_format | pubmed |
| spellingShingle | Efficient DNA Extraction Coupled with Fluorescence Loop-Mediated Isothermal Amplification (LAMP) for On-Site Early Warning Detection of Vibrio parahaemolyticus. Liu, Fengyi Geng, Qilin Sun, Xue Lin, Jie Zhu, Xuan Wang, Danyang Zhang, Xiao-Hua Hu, Jingjie Wang, Shi Bao, Lisui Lv, Jia Vibrio parahaemolyticus Nucleic Acid Amplification Techniques DNA, Bacterial Animals Sensitivity and Specificity Aquaculture Fluorescence Seawater Molecular Diagnostic Techniques Vibrio Infections Efficient DNA Extraction Coupled with Fluorescence Loop-Mediated Isothermal Amplification (LAMP) for On-Site Early Warning Detection of Vibrio parahaemolyticus. Liu, Fengyi Geng, Qilin Sun, Xue Lin, Jie Zhu, Xuan Wang, Danyang Zhang, Xiao-Hua Hu, Jingjie Wang, Shi Bao, Lisui Lv, Jia Vibrio parahaemolyticus Nucleic Acid Amplification Techniques DNA, Bacterial Animals Sensitivity and Specificity Aquaculture Fluorescence Seawater Molecular Diagnostic Techniques Vibrio Infections Vibrio parahaemolyticus (V. parahaemolyticus), a significant pathogen in aquatic products, necessitates rapid and precise detection to prevent foodborne disease outbreaks. Early detection of bacterial pathogens is crucial for effective prevention and control. In this study, we developed a field-deployable, "sample-in, answer-out" workflow that integrates efficient DNA extraction with visual fluorescence loop-mediated isothermal amplification (LAMP) for the on-site detection of V. parahaemolyticus. This extraction protocol enables efficient one-pot DNA release within 10 min, eliminating the need for additional purification steps. In addition, coupled with an optimized fluorescence LAMP assay targeting V. parahaemolyticus, the entire process-from sample to result-can be completed within approximately 50 min. The optimized assay exhibited high sensitivity, detecting as low as 1 copy/µL of plasmid DNA and 10 CFU per reaction from pure bacterial culture and spiked seawater samples. This extraction-amplification integrated approach demonstrates robust performance and high practicality for on-site early warning and pathogen monitoring in aquaculture safety settings. |
| title | Efficient DNA Extraction Coupled with Fluorescence Loop-Mediated Isothermal Amplification (LAMP) for On-Site Early Warning Detection of Vibrio parahaemolyticus. |
| topic | Vibrio parahaemolyticus Nucleic Acid Amplification Techniques DNA, Bacterial Animals Sensitivity and Specificity Aquaculture Fluorescence Seawater Molecular Diagnostic Techniques Vibrio Infections |
| url | https://pubmed.ncbi.nlm.nih.gov/42240781/ |