Enregistré dans:
| Auteurs principaux: | , , , , , , , , |
|---|---|
| Format: | Artículo científico |
| Langue: | en |
| Publié: |
Marine biotechnology (New York, N.Y.)
2026
|
| Sujets: | |
| Accès en ligne: | https://pubmed.ncbi.nlm.nih.gov/42250117/ |
| Tags: |
Ajouter un tag
Pas de tags, Soyez le premier à ajouter un tag!
|
Table des matières:
- Unveiling Complete Genome of Streptococcus agalactiae from Malaysian Aquaculture: A Closer Look at Molecular Characteristics and Phylogenomic. Zainal Fithri, Helmi Husaini Samsulrizal, Nurul Hidayah Mansor, Nur-Nazifah Hamzah, Nurasyikin Abu Halim, Noor Hanis Ridzuan, Mohd Syafiq Mohammad Abdullah, Azila Abdul Rahim, Nur Amalin Sofea Abdul Hamid, Azzmer Azzar Streptococcus agalactiae Animals Phylogeny Malaysia Genome, Bacterial Aquaculture Fish Diseases Streptococcal Infections Multilocus Sequence Typing Tilapia Drug Resistance, Bacterial Whole Genome Sequencing Streptococcus agalactiae (Group B Streptococcus, GBS) is a significant pathogen in aquaculture worldwide and is responsible for high mortality in farmed fish. Despite its regional impact, complete genome data from Malaysian isolates remain scarce. In this study, we report the first complete genome of a Malaysian S. agalactiae isolate, SA2BKE, derived from infected tilapia. Using Oxford Nanopore long-read sequencing, we assembled a 2.03 Mb circular complete genome of S. agalactiae. The functional annotation revealed 1,970 protein-coding genes and 108 RNA genes. Several antimicrobial resistance genes, including tet(M), mreA, and mprF, are associated with resistance to tetracyclines, macrolides, and peptides, respectively. Notably, there are 15 virulence-associated proteins involved in cell wall/membrane/envelope biogenesis. Multilocus sequence typing (MLST) identified SA2BKE as sequence type ST283, which has the potential to infect both fish and humans. Comparative phylogenomic analysis revealed 215 global strains positioned SA2BKE within a clade of other ST283 isolates from Asia and South America, suggesting potential transregional transmission. Pan-genome analysis identified 555 core genes shared among the analysed genomes, highlighting substantial genomic diversity within the species. Additionally, 14 mobile genetic element-associated regions were detected in SA2BKE, indicating potential genome plasticity and horizontal gene transfer events. These findings expand the genomic reference data for S. agalactiae isolates from Malaysia, contributing to regional surveillance efforts across Southeast Asia and supporting integrated disease management strategies in aquaculture.