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| Main Authors: | , , , , , , |
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| Format: | Artículo científico |
| Language: | en |
| Published: |
Tissue & cell
2026
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| Online Access: | https://pubmed.ncbi.nlm.nih.gov/42269420/ |
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| _version_ | 1868266039382376449 |
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| author | Coelho, Sofia Oliveira, Elsa Fonseca Cardoso, Margarida Lobo Xavier, Sofia Barros, Alberto Marques, C Joana Sousa, Mário |
| author_facet | Coelho, Sofia Oliveira, Elsa Fonseca Cardoso, Margarida Lobo Xavier, Sofia Barros, Alberto Marques, C Joana Sousa, Mário Coelho, Sofia Oliveira, Elsa Fonseca Cardoso, Margarida Lobo Xavier, Sofia Barros, Alberto Marques, C Joana Sousa, Mário |
| collection | PubMed - marine biology |
| contents | Quantitative prospective experimental study on time-dependent ultrastructural changes in human spermatozoa after cryopreservation. Coelho, Sofia Oliveira, Elsa Fonseca Cardoso, Margarida Lobo Xavier, Sofia Barros, Alberto Marques, C Joana Sousa, Mário Sperm cryopreservation is a common procedure in assisted reproduction (ART) laboratories. Although generally regarded as an efficient and safe technique, a decrease in the fertilization potential has been described. Hence, a detailed ultrastructural analysis of human spermatozoa might provide important insights into cellular alterations occurring during cryopreservation. To address this question, the ultrastructure of human spermatozoa was analysed, before and after cryopreservation, for the time periods of one month (1-Mo) and seven months (7-Mo), using paired, repeated measures. Semen samples were cryopreserved with a rapid freezing protocol, using a glycerol- and raffinose-containing cryopreservation medium, allocated into cryo-straws and stored in liquid nitrogen. Ultrastructural results indicate a significant increase in anomalous sperm, with changes in the head membrane and acrosomal vesicle, after both cryopreservation periods (fresh:26.8% vs. 1-Mo:52.2%, 7-Mo:57.4%, P |
| format | Artículo científico |
| id | pubmed_42269420 |
| institution | PubMed |
| language | en |
| publishDate | 2026 |
| publisher | Tissue & cell |
| record_format | pubmed |
| spellingShingle | Quantitative prospective experimental study on time-dependent ultrastructural changes in human spermatozoa after cryopreservation. Coelho, Sofia Oliveira, Elsa Fonseca Cardoso, Margarida Lobo Xavier, Sofia Barros, Alberto Marques, C Joana Sousa, Mário Quantitative prospective experimental study on time-dependent ultrastructural changes in human spermatozoa after cryopreservation. Coelho, Sofia Oliveira, Elsa Fonseca Cardoso, Margarida Lobo Xavier, Sofia Barros, Alberto Marques, C Joana Sousa, Mário Sperm cryopreservation is a common procedure in assisted reproduction (ART) laboratories. Although generally regarded as an efficient and safe technique, a decrease in the fertilization potential has been described. Hence, a detailed ultrastructural analysis of human spermatozoa might provide important insights into cellular alterations occurring during cryopreservation. To address this question, the ultrastructure of human spermatozoa was analysed, before and after cryopreservation, for the time periods of one month (1-Mo) and seven months (7-Mo), using paired, repeated measures. Semen samples were cryopreserved with a rapid freezing protocol, using a glycerol- and raffinose-containing cryopreservation medium, allocated into cryo-straws and stored in liquid nitrogen. Ultrastructural results indicate a significant increase in anomalous sperm, with changes in the head membrane and acrosomal vesicle, after both cryopreservation periods (fresh:26.8% vs. 1-Mo:52.2%, 7-Mo:57.4%, P |
| title | Quantitative prospective experimental study on time-dependent ultrastructural changes in human spermatozoa after cryopreservation. |
| url | https://pubmed.ncbi.nlm.nih.gov/42269420/ |