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Main Authors: Huang, Jinwei, Xu, Pingrui, Xiao, Yongshuang, Li, Jun
Format: Artículo científico
Language:en
Published: Biology 2026
Online Access:https://pubmed.ncbi.nlm.nih.gov/42274508/
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author Huang, Jinwei
Xu, Pingrui
Xiao, Yongshuang
Li, Jun
author_facet Huang, Jinwei
Xu, Pingrui
Xiao, Yongshuang
Li, Jun
Huang, Jinwei
Xu, Pingrui
Xiao, Yongshuang
Li, Jun
collection PubMed - marine biology
contents GnRH Regulates Sex Differentiation in Through TGF-β/MAPK Signaling Pathways. Huang, Jinwei Xu, Pingrui Xiao, Yongshuang Li, Jun Sex determination and gonadal differentiation are essential for vertebrate development and reproduction. Fish, with both genetic and environmental sex-determining mechanisms, serve as ideal models for exploring environmental effects on gonadal development. The hypothalamic-pituitary-gonadal (HPG) axis is involved in sex differentiation, but its role as a key mediator in temperature-dependent sex differentiation remains unclear. is an economically important marine fish species along the northern coast of China. It exhibits obvious sexual growth dimorphism and typical temperature-dependent sex differentiation, making it an excellent model for investigating the mechanism of sex differentiation. In this study, individuals of at the sex differentiation stage were treated with a GnRH receptor antagonist and subjected to transcriptome sequencing analysis. The results revealed that GnRH may regulate sex differentiation through steroid biosynthesis, TGF-β and MAPK signaling pathways. We identified several key genes, including , , , , , and , which may affect gonadal differentiation by acting on core sex-determining genes and steroidogenesis. In conclusion, GnRH precisely mediates the process of sex differentiation by modulating the TGF-β signaling pathway, the MAPK signaling pathway, and local gonadal steroidogenic networks.
format Artículo científico
id pubmed_42274508
institution PubMed
language en
publishDate 2026
publisher Biology
record_format pubmed
spellingShingle GnRH Regulates Sex Differentiation in Through TGF-β/MAPK Signaling Pathways.
Huang, Jinwei
Xu, Pingrui
Xiao, Yongshuang
Li, Jun
GnRH Regulates Sex Differentiation in Through TGF-β/MAPK Signaling Pathways. Huang, Jinwei Xu, Pingrui Xiao, Yongshuang Li, Jun Sex determination and gonadal differentiation are essential for vertebrate development and reproduction. Fish, with both genetic and environmental sex-determining mechanisms, serve as ideal models for exploring environmental effects on gonadal development. The hypothalamic-pituitary-gonadal (HPG) axis is involved in sex differentiation, but its role as a key mediator in temperature-dependent sex differentiation remains unclear. is an economically important marine fish species along the northern coast of China. It exhibits obvious sexual growth dimorphism and typical temperature-dependent sex differentiation, making it an excellent model for investigating the mechanism of sex differentiation. In this study, individuals of at the sex differentiation stage were treated with a GnRH receptor antagonist and subjected to transcriptome sequencing analysis. The results revealed that GnRH may regulate sex differentiation through steroid biosynthesis, TGF-β and MAPK signaling pathways. We identified several key genes, including , , , , , and , which may affect gonadal differentiation by acting on core sex-determining genes and steroidogenesis. In conclusion, GnRH precisely mediates the process of sex differentiation by modulating the TGF-β signaling pathway, the MAPK signaling pathway, and local gonadal steroidogenic networks.
title GnRH Regulates Sex Differentiation in Through TGF-β/MAPK Signaling Pathways.
url https://pubmed.ncbi.nlm.nih.gov/42274508/