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| Main Authors: | , , , , , , , , , |
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| Format: | Artículo científico |
| Language: | en |
| Published: |
Research square
2026
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| Online Access: | https://pubmed.ncbi.nlm.nih.gov/42282017/ |
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| _version_ | 1868266037091237888 |
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| author | Chaulagain, Diptee Paul, Bristi Leslie, Skylar Artigues-Lleixà, Maria Cros, Maria Pol Toloza, Lorena Tang, Zeyu Hoover, Adam Güell, Marc Karig, David |
| author_facet | Chaulagain, Diptee Paul, Bristi Leslie, Skylar Artigues-Lleixà, Maria Cros, Maria Pol Toloza, Lorena Tang, Zeyu Hoover, Adam Güell, Marc Karig, David Chaulagain, Diptee Paul, Bristi Leslie, Skylar Artigues-Lleixà, Maria Cros, Maria Pol Toloza, Lorena Tang, Zeyu Hoover, Adam Güell, Marc Karig, David |
| collection | PubMed - marine biology |
| contents | Rational assembly of synthetic marine biofilm community with chitinase production. Chaulagain, Diptee Paul, Bristi Leslie, Skylar Artigues-Lleixà, Maria Cros, Maria Pol Toloza, Lorena Tang, Zeyu Hoover, Adam Güell, Marc Karig, David Highly diverse multispecies biofilms are ubiquitous in microbial ecosystems; however, our current understanding of biofilm dynamics is limited to single species or low richness studies. We aimed to design a multispecies biofilm with a targeted function, chitinase production, using natural marine bacteria. We present a top-down assembly approach to design functional biofilm communities. Using our method, we found that final community membership was established within 24 hours, regardless of nutrient availability. However, cultivation in nutrient-rich media enabled rapid identification of the competitive dominant taxon, , among the 17 initial isolates used in the assembly. By repeating community assembly in a low-nutrient medium without these highly competitive taxa, we achieved the highest species diversity in the biofilm. The resulting multispecies biofilm exhibited chitinase production and maintained ~ 50% persistence during peak invasion. By comparison, a single species chitinase-producing biofilm formed lower biomass and suffered higher displacement during invasion. Importantly, one member that withstood invasion challenge in the multispecies community was completely undetectable at seven days post-invasion as a single species biofilm, indicating collective invasion resilience in the multispecies community. Further evidence of cooperation for coexistence is supported by increased β-N-acetylglucosaminidase, enzyme that hydrolyzes chitin oligomers, in the 14-member community at later timepoints, while the detected exochitinase activity remained stable. Our findings present a streamlined strategy to assemble diverse and functional biofilm communities for targeted biofilm engineering in marine and applied microbiome contexts, and our achievement of engineered function using natural bacteria offers a powerful complement to synthetic biology. |
| format | Artículo científico |
| id | pubmed_42282017 |
| institution | PubMed |
| language | en |
| publishDate | 2026 |
| publisher | Research square |
| record_format | pubmed |
| spellingShingle | Rational assembly of synthetic marine biofilm community with chitinase production. Chaulagain, Diptee Paul, Bristi Leslie, Skylar Artigues-Lleixà, Maria Cros, Maria Pol Toloza, Lorena Tang, Zeyu Hoover, Adam Güell, Marc Karig, David Rational assembly of synthetic marine biofilm community with chitinase production. Chaulagain, Diptee Paul, Bristi Leslie, Skylar Artigues-Lleixà, Maria Cros, Maria Pol Toloza, Lorena Tang, Zeyu Hoover, Adam Güell, Marc Karig, David Highly diverse multispecies biofilms are ubiquitous in microbial ecosystems; however, our current understanding of biofilm dynamics is limited to single species or low richness studies. We aimed to design a multispecies biofilm with a targeted function, chitinase production, using natural marine bacteria. We present a top-down assembly approach to design functional biofilm communities. Using our method, we found that final community membership was established within 24 hours, regardless of nutrient availability. However, cultivation in nutrient-rich media enabled rapid identification of the competitive dominant taxon, , among the 17 initial isolates used in the assembly. By repeating community assembly in a low-nutrient medium without these highly competitive taxa, we achieved the highest species diversity in the biofilm. The resulting multispecies biofilm exhibited chitinase production and maintained ~ 50% persistence during peak invasion. By comparison, a single species chitinase-producing biofilm formed lower biomass and suffered higher displacement during invasion. Importantly, one member that withstood invasion challenge in the multispecies community was completely undetectable at seven days post-invasion as a single species biofilm, indicating collective invasion resilience in the multispecies community. Further evidence of cooperation for coexistence is supported by increased β-N-acetylglucosaminidase, enzyme that hydrolyzes chitin oligomers, in the 14-member community at later timepoints, while the detected exochitinase activity remained stable. Our findings present a streamlined strategy to assemble diverse and functional biofilm communities for targeted biofilm engineering in marine and applied microbiome contexts, and our achievement of engineered function using natural bacteria offers a powerful complement to synthetic biology. |
| title | Rational assembly of synthetic marine biofilm community with chitinase production. |
| url | https://pubmed.ncbi.nlm.nih.gov/42282017/ |