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| Auteurs principaux: | , , , , , , |
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| Format: | Artículo científico |
| Langue: | en |
| Publié: |
Marine biotechnology (New York, N.Y.)
2026
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| Sujets: | |
| Accès en ligne: | https://pubmed.ncbi.nlm.nih.gov/42287469/ |
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| _version_ | 1868266037083897856 |
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| author | Zou, Ailing Yuan, Yuying Zhu, Lingling Zhang, Yu Xiao, Lanfei Wen, Xiaobo Lin, Fan |
| author_facet | Zou, Ailing Yuan, Yuying Zhu, Lingling Zhang, Yu Xiao, Lanfei Wen, Xiaobo Lin, Fan Zou, Ailing Yuan, Yuying Zhu, Lingling Zhang, Yu Xiao, Lanfei Wen, Xiaobo Lin, Fan |
| collection | PubMed - marine biology |
| contents | Characterization of the ctgf Promoter from Nibea coibor and its Response to TGF-β1 During Collagen Synthesis in Swim Bladder Cells. Zou, Ailing Yuan, Yuying Zhu, Lingling Zhang, Yu Xiao, Lanfei Wen, Xiaobo Lin, Fan Animals Connective Tissue Growth Factor Promoter Regions, Genetic Transforming Growth Factor beta1 Collagen Air Sacs Perciformes Gene Expression Regulation Base Sequence Mutagenesis, Site-Directed Fish Proteins Binding Sites NF-kappa B Connective tissue growth factor (ctgf) is a multifunctional protein that plays a crucial role in promoting collagen (the most abundant protein in organism) synthesis in vertebrates. However, its transcriptional regulation mechanism is poorly understood, especially in teleosts. In this study, we characterized the ctgf promoter (3,185 bp) from a marine teleost, the chu's croaker (Nibea coibor), and elucidated its response to TGF-β1 during collagen synthesis in swim bladder cells. Bioinformatics analysis revealed conserved cis-elements (TATA box, SP-1, Smad-3, NF-κB) within the promoter. Truncation assays identified a core functional region (-785 ~ + 166 bp). Notably, site-directed mutagenesis showed that mutation of SP-1 binding site enhanced the promoter activity, while mutations in binding sites of Smad-3 (-290 bp) and NF-κB (-262 bp) suppressed its activity. To demonstrate its correlation with collagen synthesis, swim bladder cells stably transfected with ctgf promoter drived EGFP vector (pctgf-EGFP) was constructed. It was further found that the promoter activity was up-regulated in concert with collagen synthesis promoted by TGF-β1 in swim bladder cells. These findings could advance our understanding of the transcriptional regulation of ctgf, and establish a technical platform for further screening natural compounds promoting collagen synthesis in fish. |
| format | Artículo científico |
| id | pubmed_42287469 |
| institution | PubMed |
| language | en |
| publishDate | 2026 |
| publisher | Marine biotechnology (New York, N.Y.) |
| record_format | pubmed |
| spellingShingle | Characterization of the ctgf Promoter from Nibea coibor and its Response to TGF-β1 During Collagen Synthesis in Swim Bladder Cells. Zou, Ailing Yuan, Yuying Zhu, Lingling Zhang, Yu Xiao, Lanfei Wen, Xiaobo Lin, Fan Animals Connective Tissue Growth Factor Promoter Regions, Genetic Transforming Growth Factor beta1 Collagen Air Sacs Perciformes Gene Expression Regulation Base Sequence Mutagenesis, Site-Directed Fish Proteins Binding Sites NF-kappa B Characterization of the ctgf Promoter from Nibea coibor and its Response to TGF-β1 During Collagen Synthesis in Swim Bladder Cells. Zou, Ailing Yuan, Yuying Zhu, Lingling Zhang, Yu Xiao, Lanfei Wen, Xiaobo Lin, Fan Animals Connective Tissue Growth Factor Promoter Regions, Genetic Transforming Growth Factor beta1 Collagen Air Sacs Perciformes Gene Expression Regulation Base Sequence Mutagenesis, Site-Directed Fish Proteins Binding Sites NF-kappa B Connective tissue growth factor (ctgf) is a multifunctional protein that plays a crucial role in promoting collagen (the most abundant protein in organism) synthesis in vertebrates. However, its transcriptional regulation mechanism is poorly understood, especially in teleosts. In this study, we characterized the ctgf promoter (3,185 bp) from a marine teleost, the chu's croaker (Nibea coibor), and elucidated its response to TGF-β1 during collagen synthesis in swim bladder cells. Bioinformatics analysis revealed conserved cis-elements (TATA box, SP-1, Smad-3, NF-κB) within the promoter. Truncation assays identified a core functional region (-785 ~ + 166 bp). Notably, site-directed mutagenesis showed that mutation of SP-1 binding site enhanced the promoter activity, while mutations in binding sites of Smad-3 (-290 bp) and NF-κB (-262 bp) suppressed its activity. To demonstrate its correlation with collagen synthesis, swim bladder cells stably transfected with ctgf promoter drived EGFP vector (pctgf-EGFP) was constructed. It was further found that the promoter activity was up-regulated in concert with collagen synthesis promoted by TGF-β1 in swim bladder cells. These findings could advance our understanding of the transcriptional regulation of ctgf, and establish a technical platform for further screening natural compounds promoting collagen synthesis in fish. |
| title | Characterization of the ctgf Promoter from Nibea coibor and its Response to TGF-β1 During Collagen Synthesis in Swim Bladder Cells. |
| topic | Animals Connective Tissue Growth Factor Promoter Regions, Genetic Transforming Growth Factor beta1 Collagen Air Sacs Perciformes Gene Expression Regulation Base Sequence Mutagenesis, Site-Directed Fish Proteins Binding Sites NF-kappa B |
| url | https://pubmed.ncbi.nlm.nih.gov/42287469/ |