TBK1 associated with TRIF and TRAF3 related IRF3, IRF7, and type I IFN signaling in large yellow croaker Larimichthys crocea.
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| Main Authors: | , , , , , , , , , , |
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| Format: | Artículo científico |
| Language: | en |
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Fish & shellfish immunology
2026
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| _version_ | 1868266037060829185 |
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| author | Li, Ying Zha, Wen Qi Cui, Wen Feng Li, Meng Jia Chen, Yan Mi Na Huang, Qiu Ye Yang, Qiao Yun Shen, Ying Jia Wang, Yi Lei Jiang, Yong Hua Zou, Peng Fei |
| author_facet | Li, Ying Zha, Wen Qi Cui, Wen Feng Li, Meng Jia Chen, Yan Mi Na Huang, Qiu Ye Yang, Qiao Yun Shen, Ying Jia Wang, Yi Lei Jiang, Yong Hua Zou, Peng Fei Li, Ying Zha, Wen Qi Cui, Wen Feng Li, Meng Jia Chen, Yan Mi Na Huang, Qiu Ye Yang, Qiao Yun Shen, Ying Jia Wang, Yi Lei Jiang, Yong Hua Zou, Peng Fei |
| collection | PubMed - marine biology |
| contents | TBK1 associated with TRIF and TRAF3 related IRF3, IRF7, and type I IFN signaling in large yellow croaker Larimichthys crocea. Li, Ying Zha, Wen Qi Cui, Wen Feng Li, Meng Jia Chen, Yan Mi Na Huang, Qiu Ye Yang, Qiao Yun Shen, Ying Jia Wang, Yi Lei Jiang, Yong Hua Zou, Peng Fei TANK-binding kinase 1 (TBK1) plays a central role in the host's defense system. In this research, we identified and analyzed the TBK1 ortholog from large yellow croaker (Larimichthys crocea) and investigated its function in the host innate immunity. The obtained ORF of Lc-TBK1 is 2,172 bp and encoding a 723 aa protein. Subcellular localization analysis demonstrated that Lc-TBK1 predominantly resides in the cytoplasm. Lc-TBK1 showed broad expression across multiple tissues, and its transcript abundance was particularly high in the muscle. Its transcription was significantly upregulated after challenge with poly I:C, LPS, PGN, and Pseudomonas plecoglossicida. Overexpression of Lc-TBK1 activated IRF3, IRF7, and IFNd promoters, and such promoter activation were enhanced when Lc-TBK1 co-expressed with Lc-TRAF3 and Lc-TRIF. Co-immunoprecipitation assays confirmed direct interactions between Lc-TBK1 and several key adaptors, including Lc-TRIF, Lc-MAVS_tv1, Lc-MAVS_tv2, Lc-TRAF3, and Lc-TRAF6. Subsequent co-expression analyses revealed adaptor-specific modulation of antiviral gene transcription: Lc-TRIF and Lc-TRAF3 synergistically enhanced RSAD2, IRF3, and IRF7 expression, whereas Lc-MAVS isoforms attenuated these responses. Overall, our findings demonstrate that Lc-TBK1 signaling is differentially regulated by specific adaptor proteins, providing new insights into the evolutionary conservation and diversification of antiviral immune mechanisms in teleost. |
| format | Artículo científico |
| id | pubmed_42297082 |
| institution | PubMed |
| language | en |
| publishDate | 2026 |
| publisher | Fish & shellfish immunology |
| record_format | pubmed |
| spellingShingle | TBK1 associated with TRIF and TRAF3 related IRF3, IRF7, and type I IFN signaling in large yellow croaker Larimichthys crocea. Li, Ying Zha, Wen Qi Cui, Wen Feng Li, Meng Jia Chen, Yan Mi Na Huang, Qiu Ye Yang, Qiao Yun Shen, Ying Jia Wang, Yi Lei Jiang, Yong Hua Zou, Peng Fei TBK1 associated with TRIF and TRAF3 related IRF3, IRF7, and type I IFN signaling in large yellow croaker Larimichthys crocea. Li, Ying Zha, Wen Qi Cui, Wen Feng Li, Meng Jia Chen, Yan Mi Na Huang, Qiu Ye Yang, Qiao Yun Shen, Ying Jia Wang, Yi Lei Jiang, Yong Hua Zou, Peng Fei TANK-binding kinase 1 (TBK1) plays a central role in the host's defense system. In this research, we identified and analyzed the TBK1 ortholog from large yellow croaker (Larimichthys crocea) and investigated its function in the host innate immunity. The obtained ORF of Lc-TBK1 is 2,172 bp and encoding a 723 aa protein. Subcellular localization analysis demonstrated that Lc-TBK1 predominantly resides in the cytoplasm. Lc-TBK1 showed broad expression across multiple tissues, and its transcript abundance was particularly high in the muscle. Its transcription was significantly upregulated after challenge with poly I:C, LPS, PGN, and Pseudomonas plecoglossicida. Overexpression of Lc-TBK1 activated IRF3, IRF7, and IFNd promoters, and such promoter activation were enhanced when Lc-TBK1 co-expressed with Lc-TRAF3 and Lc-TRIF. Co-immunoprecipitation assays confirmed direct interactions between Lc-TBK1 and several key adaptors, including Lc-TRIF, Lc-MAVS_tv1, Lc-MAVS_tv2, Lc-TRAF3, and Lc-TRAF6. Subsequent co-expression analyses revealed adaptor-specific modulation of antiviral gene transcription: Lc-TRIF and Lc-TRAF3 synergistically enhanced RSAD2, IRF3, and IRF7 expression, whereas Lc-MAVS isoforms attenuated these responses. Overall, our findings demonstrate that Lc-TBK1 signaling is differentially regulated by specific adaptor proteins, providing new insights into the evolutionary conservation and diversification of antiviral immune mechanisms in teleost. |
| title | TBK1 associated with TRIF and TRAF3 related IRF3, IRF7, and type I IFN signaling in large yellow croaker Larimichthys crocea. |
| url | https://pubmed.ncbi.nlm.nih.gov/42297082/ |