An invariant VLR-like receptor refines the T-like cell framework in lamprey.

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Autores principales: Geng, Ming, Li, Kang, Zhu, Yating, Zou, Baohua, Li, Jun, Chen, Haokai, Fang, Zhichao, Jiao, Xinying, Zhai, Xue, Hu, Peng, Wei, Xiumei, Pang, Yue, Yang, Jialong
Formato: Artículo científico
Lenguaje:en
Publicado: Nature communications 2026
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author Geng, Ming
Li, Kang
Zhu, Yating
Zou, Baohua
Li, Jun
Chen, Haokai
Fang, Zhichao
Jiao, Xinying
Zhai, Xue
Hu, Peng
Wei, Xiumei
Pang, Yue
Yang, Jialong
author_facet Geng, Ming
Li, Kang
Zhu, Yating
Zou, Baohua
Li, Jun
Chen, Haokai
Fang, Zhichao
Jiao, Xinying
Zhai, Xue
Hu, Peng
Wei, Xiumei
Pang, Yue
Yang, Jialong
Geng, Ming
Li, Kang
Zhu, Yating
Zou, Baohua
Li, Jun
Chen, Haokai
Fang, Zhichao
Jiao, Xinying
Zhai, Xue
Hu, Peng
Wei, Xiumei
Pang, Yue
Yang, Jialong
collection PubMed - marine biology
contents An invariant VLR-like receptor refines the T-like cell framework in lamprey. Geng, Ming Li, Kang Zhu, Yating Zou, Baohua Li, Jun Chen, Haokai Fang, Zhichao Jiao, Xinying Zhai, Xue Hu, Peng Wei, Xiumei Pang, Yue Yang, Jialong Jawless vertebrates employ variable lymphocyte receptors (VLRs) to mount adaptive immunity. Although six VLRs (VLRA-F) and T/B-like lymphocytes are identified in lampreys, it remains unclear how VLR diversity is organized at the cellular level and whether individual lymphocytes may integrate multiple VLRs. Using single-cell transcriptomics, we identify a germline-encoded, mature, non-diversified VLR-like receptor (VLR-like) in lamprey Lethenteron reissneri. VLR-like retains structural features of canonical VLRs, and is a GPI-anchored receptor expressed by T-like cells. We generate a VLR-like monoclonal antibody, enabling the identification of VLR-like lymphocytes across multiple tissues. Upon PHA stimulation, VLR-like lymphocytes exhibit robust ERK and JNK phosphorylation and proliferation, consistent with T-like cell features. In parallel, VLR-like cells fail to phagocytose, present antigen, or secrete soluble VLR-like, indicating the absence of B-like characteristics. Notably, VLR-like cells are distinct from VLRA lymphocytes but comprise both VLR-likeVLRC and VLR-likeVLRC populations. Consistently, VLR-like interacts with VLRC but not VLRA. Together, we demonstrate that VLR-like defines a distinct T-like population with heterogeneous VLRC co-expression, and suggest a mode of receptor organization in which an invariant VLR-like coordinates a diversified VLR within an individual T-like cell, potentially contributing to adaptive immunity in lamprey.
format Artículo científico
id pubmed_42297841
institution PubMed
language en
publishDate 2026
publisher Nature communications
record_format pubmed
spellingShingle An invariant VLR-like receptor refines the T-like cell framework in lamprey.
Geng, Ming
Li, Kang
Zhu, Yating
Zou, Baohua
Li, Jun
Chen, Haokai
Fang, Zhichao
Jiao, Xinying
Zhai, Xue
Hu, Peng
Wei, Xiumei
Pang, Yue
Yang, Jialong
An invariant VLR-like receptor refines the T-like cell framework in lamprey. Geng, Ming Li, Kang Zhu, Yating Zou, Baohua Li, Jun Chen, Haokai Fang, Zhichao Jiao, Xinying Zhai, Xue Hu, Peng Wei, Xiumei Pang, Yue Yang, Jialong Jawless vertebrates employ variable lymphocyte receptors (VLRs) to mount adaptive immunity. Although six VLRs (VLRA-F) and T/B-like lymphocytes are identified in lampreys, it remains unclear how VLR diversity is organized at the cellular level and whether individual lymphocytes may integrate multiple VLRs. Using single-cell transcriptomics, we identify a germline-encoded, mature, non-diversified VLR-like receptor (VLR-like) in lamprey Lethenteron reissneri. VLR-like retains structural features of canonical VLRs, and is a GPI-anchored receptor expressed by T-like cells. We generate a VLR-like monoclonal antibody, enabling the identification of VLR-like lymphocytes across multiple tissues. Upon PHA stimulation, VLR-like lymphocytes exhibit robust ERK and JNK phosphorylation and proliferation, consistent with T-like cell features. In parallel, VLR-like cells fail to phagocytose, present antigen, or secrete soluble VLR-like, indicating the absence of B-like characteristics. Notably, VLR-like cells are distinct from VLRA lymphocytes but comprise both VLR-likeVLRC and VLR-likeVLRC populations. Consistently, VLR-like interacts with VLRC but not VLRA. Together, we demonstrate that VLR-like defines a distinct T-like population with heterogeneous VLRC co-expression, and suggest a mode of receptor organization in which an invariant VLR-like coordinates a diversified VLR within an individual T-like cell, potentially contributing to adaptive immunity in lamprey.
title An invariant VLR-like receptor refines the T-like cell framework in lamprey.
url https://pubmed.ncbi.nlm.nih.gov/42297841/