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| Main Authors: | , , , , , , |
|---|---|
| Format: | Artículo científico |
| Language: | en |
| Published: |
Communications biology
2026
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| Online Access: | https://pubmed.ncbi.nlm.nih.gov/42298151/ |
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Table of Contents:
- Efficient recovery of zebrafish maternal-zygotic mutants from in vivo expanded and cryopreserved germline stem cells. Wang, Yaqing Qu, Rui Wang, Xiaosi Hao, Yongkang Deng, Jiajie Wang, Houpeng Sun, Yonghua Long-term preservation of genetic lines is crucial for animal research. Current methods heavily rely on cryopreservation of haploid sperm, whereas diploid germline stem cells (GSCs) may provide a superior alternative. Here, using zebrafish, we establish an integrated platform combining in vivo expansion of GSCs (eGSCs), cryopreservation and direct genetic recovery. The eGSCs are amplified in cyp11a2 mutants and exhibit a total number of about 300-fold higher than conventional GSCs after cryopreservation and thawing. The eGSCs achieve a success rate of 82% after transplantation, compared with less than 1% for conventional GSCs. Furthermore, we develop the GSC-deficient nanos2 mutant as an ideal host for eGSC transplantation, enabling efficient production of both sperm and oocytes within a single maturation period. This allows rapid recovery of maternal-zygotic mutants directly in the F1 generation. Overall, our strategy provides a robust platform for preserving and restoring zebrafish homozygous mutants, opening new opportunities for zebrafish research.