Evaluation of genotoxic activity of maleic hydrazide, ethyl methane sulfonate, and N-nitroso diethylamine in Tradescantia
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Instituto Nacional de Salud Pública
2001
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| author | Carlos Alvarez |
| author_facet | Carlos Alvarez |
| contents | Evaluation of genotoxic activity of maleic hydrazide, ethyl methane sulfonate, and N-nitroso diethylamine in Tradescantia Carlos Alvarez Anne Santerre Guillermo Zúñiga-González Olivia Torres Eduardo Padilla Alfredo Feria Salud Mexico Comet assay Tradescantia genotoxicity test Key words: environmental mutagens Objective. To assess the genotoxic activity of N-nitrosodiethylamine (NDEA), maleic hydrazide (MH), and ethylmethane sulfonate (EMS) using two systems: the comet assayon nuclei from Tradescantia, and the pink mutation teston Tradescantia staminal hairs (clone 4430). Material andMethods. Tradescantia cups was obtained from Laboratoriode Citogenética y Mutagénesis del Centro de Cienciasde la Atmósfera de la Universidad Nacional Autónoma deMéxico and treated with: N-nitroso diethylamine (NDEA)at 1, 5, 10 mM, maleic hidrazide (MH) at 1, 5, 10 mM andethyl methane sulfonate (EMS) at 15, 30 and 45 mM;and used in both pink mutation assay and comet assay usingcellular nuclei from Tradescantia staminal hairs. The observationof staminal hair was realized along eight days (6-14)after treatment), flowers produced day 14 after treatmentwere utilized done according to Underbrink. In previousreports on plants, were comet assay was used, breaking cellularwall and separating by centrifugation gradient are necessary.Here, nuclei from staminal hairs were obtained bysquashing the cells (is not necessary to utilize to break specialprocedure cellular wall), collected using a nylon meshof 80Mm and next the comet assay was applied. Students Ttest was the statistical test used for analyzing the cometassay data. Results. Both assays showed a great sensitivityto the studied mutagens. A relationship between thedose-pink event and the dose-tail length was evident. Eventhough the Tradescantia mutation assay is a sensitive testwith MH and EMS, low doses of NDEA were not able toinduce a significant increase in the pink event frequencies;however, the comet assay was able to detect the mutageniceffect of NDEA at the same dose. Thus, it is clear that thecomet assay is highly sensitive to the lowest dose of chemicalmutagens. Conclusions. The comet assay on nucleifrom Tradescantia staminal hairs is a useful tool to monitorgenotoxic agents; it is simple, highly sensitive, and faster thanthe pink mutation test. The English version of this paper isavailable too at: http://www.insp.mx/salud/index.html 2001 artículo científico 0036-3634 https://www.redalyc.org/articulo.oa?id=10643609 en http://www.redalyc.org/revista.oa?id=106 Salud Pública de México application/pdf Instituto Nacional de Salud Pública Salud Pública de México (México) Num.6 Vol.43 |
| format | Artículo científico |
| id | redalyc_10643609 |
| institution | Redalyc |
| language | en |
| publishDate | 2001 |
| publisher | Instituto Nacional de Salud Pública |
| spellingShingle | Evaluation of genotoxic activity of maleic hydrazide, ethyl methane sulfonate, and N-nitroso diethylamine in Tradescantia Carlos Alvarez Salud Mexico Comet assay Tradescantia genotoxicity test Key words: environmental mutagens Evaluation of genotoxic activity of maleic hydrazide, ethyl methane sulfonate, and N-nitroso diethylamine in Tradescantia Carlos Alvarez Anne Santerre Guillermo Zúñiga-González Olivia Torres Eduardo Padilla Alfredo Feria Salud Mexico Comet assay Tradescantia genotoxicity test Key words: environmental mutagens Objective. To assess the genotoxic activity of N-nitrosodiethylamine (NDEA), maleic hydrazide (MH), and ethylmethane sulfonate (EMS) using two systems: the comet assayon nuclei from Tradescantia, and the pink mutation teston Tradescantia staminal hairs (clone 4430). Material andMethods. Tradescantia cups was obtained from Laboratoriode Citogenética y Mutagénesis del Centro de Cienciasde la Atmósfera de la Universidad Nacional Autónoma deMéxico and treated with: N-nitroso diethylamine (NDEA)at 1, 5, 10 mM, maleic hidrazide (MH) at 1, 5, 10 mM andethyl methane sulfonate (EMS) at 15, 30 and 45 mM;and used in both pink mutation assay and comet assay usingcellular nuclei from Tradescantia staminal hairs. The observationof staminal hair was realized along eight days (6-14)after treatment), flowers produced day 14 after treatmentwere utilized done according to Underbrink. In previousreports on plants, were comet assay was used, breaking cellularwall and separating by centrifugation gradient are necessary.Here, nuclei from staminal hairs were obtained bysquashing the cells (is not necessary to utilize to break specialprocedure cellular wall), collected using a nylon meshof 80Mm and next the comet assay was applied. Students Ttest was the statistical test used for analyzing the cometassay data. Results. Both assays showed a great sensitivityto the studied mutagens. A relationship between thedose-pink event and the dose-tail length was evident. Eventhough the Tradescantia mutation assay is a sensitive testwith MH and EMS, low doses of NDEA were not able toinduce a significant increase in the pink event frequencies;however, the comet assay was able to detect the mutageniceffect of NDEA at the same dose. Thus, it is clear that thecomet assay is highly sensitive to the lowest dose of chemicalmutagens. Conclusions. The comet assay on nucleifrom Tradescantia staminal hairs is a useful tool to monitorgenotoxic agents; it is simple, highly sensitive, and faster thanthe pink mutation test. The English version of this paper isavailable too at: http://www.insp.mx/salud/index.html 2001 artículo científico 0036-3634 https://www.redalyc.org/articulo.oa?id=10643609 en http://www.redalyc.org/revista.oa?id=106 Salud Pública de México application/pdf Instituto Nacional de Salud Pública Salud Pública de México (México) Num.6 Vol.43 |
| title | Evaluation of genotoxic activity of maleic hydrazide, ethyl methane sulfonate, and N-nitroso diethylamine in Tradescantia |
| topic | Salud Mexico Comet assay Tradescantia genotoxicity test Key words: environmental mutagens |
| url | https://www.redalyc.org/articulo.oa?id=10643609 |