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Main Author:	José Luis Rosales-Encina
Format:	Artículo científico
Language:	es
Published:	Instituto Nacional de Salud Pública 2007
Subjects:	Salud PCR ELISA México Nuevo León Trypanosoma cruzi
Online Access:	https://www.redalyc.org/articulo.oa?id=10649106
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Prevalencia de Trypanosoma cruzi en triatominos silvestres de Nuevo León, México José Luis Rosales-Encina Zinnia Judith Molina-Garza Lucio Galaviz-Silva Daniel Molina-Garza Salud PCR ELISA México Nuevo León Trypanosoma cruzi Objetivo. Determinar la prevalencia de Trypanosoma cruzien triatominos Triatoma gerstaeckeri de Nuevo León, mediantela estandarización de una técnica inmunoenzimática.Material y métodos. Se colectaron 52 chinches en GeneralTerán, N.L. desde julio hasta septiembre de 2005, lascuales se analizaron por microscopía óptica (MO) y reacciónen cadena de la polimerasa (PCR) como estándares dereferencia para establecer una técnica de detección del parásitopor el ensayo inmunoabsorbente ligado a enzimas(ELISA). Resultados. Por MO y PCR, 31 triatominos fueronpositivos y 21 negativos; por ELISA los resultados difierencon 27 positivos y 25 negativos (100% de especificidad, 87%de sensibilidad, 84% de valor predictivo negativo y 100% devalor predictivo positivo). Por MO y PCR, la prevalenciade triatominos infectados fue de 59.61% y por ELISA, de51.92%; por lo que se confirmó la utilidad, rapidez y elevadoíndice de confianza del ensayo de ELISA. Conclusión. Losresultados obtenidos por la técnica de ELISA son útiles paraenfocar los nuevos estudios epidemiológicos con un mayornúmero de vectores, ya que fue posible analizar simultáneamentela mayor cantidad de muestras, con niveles de sensibilidady especificidad elevados y a menor costo que el PCR;por lo tanto, se recomienda para programas de vigilanciaepidemiológica preventiva como primera prueba de tamizaje,antes de realizar análisis confirmatorios por PCR. 2007 artículo científico 0036-3634 https://www.redalyc.org/articulo.oa?id=10649106 es http://www.redalyc.org/revista.oa?id=106 Salud Pública de México application/pdf Instituto Nacional de Salud Pública Salud Pública de México (México) Num.1 Vol.49

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// Anecdotal testing Today and Yesterday seems to indicate destroy() // is called by the garbage collector and everything is good. // Something to keep in mind though. return 0; } /** * Write function that is called when session data is to be saved. * * @param string $sessId The current session ID * @param string $data The session data to write * * @return bool */ public function write($sessId, $data): bool { if ($this->writesDisabled) { return true; } return $this->saveSession($sessId, $data); } /** * A function that is called internally when session data is to be saved. * * @param string $sessId The current session ID * @param string $data The session data to write * * @return bool */ abstract protected function saveSession($sessId, $data): bool; }

* * @return void */ public function writeClose() { // The assumption is that we're using PHP's ext/session. // session_write_close() will actually overwrite $_SESSION with an // empty array on completion -- which leads to a mismatch between what // is in the storage object and $_SESSION. To get around this, we // temporarily reset $_SESSION to an array, and then re-link it to // the storage object. // // Additionally, while you _can_ write to $_SESSION following a // session_write_close() operation, no changes made to it will be // flushed to the session handler. As such, we now mark the storage // object isImmutable. $storage = $this->getStorage(); if (! $storage->isImmutable()) { $_SESSION = $storage->toArray(true); session_write_close(); $storage->fromArray($_SESSION); $storage->markImmutable(); } } /** * Attempt to set the session name * * If the session has already been started, or if the name provided fails * validation, an exception will be raised. * * @param string $name * @return SessionManager * @throws Exception\InvalidArgumentException */ public function setName($name) { if ($this->sessionExists()) { throw new Exception\InvalidArgumentException( 'Cannot set session name after a session has already started'

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