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| Format: | Artículo científico |
| Language: | en |
| Published: |
Instituto Nacional de Salud Pública
2007
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| Online Access: | https://www.redalyc.org/articulo.oa?id=10649608 |
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Table of Contents:
- SHV-type Extended-spectrum ß-lactamase (ESBL) are encoded in related plasmids from enterobacteria clinical isolates from Mexico Ulises Garza-Ramos Esperanza Martínez-Romero Jesús Silva-Sánchez Salud SHV ESBL Mexico plasmid Enterobacteria Objective: In this work we report the molecular characterization of ß-lactam antibiotics resistance conferred by genes contained in plasmids from enterobacteria producing extended-spectrum ß-lactamases (ESBL). Material and Methods: Fourteen enterobacterial clinical isolates selected from a group of strains obtained from seven different hospitals in Mexico during 1990-1992 and 1996-1998 were analyzed at the Bacterial Resistance Laboratory (National Institute Public Health, Cuernavaca). Molecular characterizationincluded PFGE, IEF of ß-lactamases, bacterial conjugation, PCR amplification and DNA sequencing, plasmid extractionand restriction. Results: Isolates were genetically unrelated. ESBL identified were SHV-2 (5/14) and SHV-5 (9/14) type.Cephalosporin-resistance was transferable in 9 of 14 (64%) clinical isolates with only one conjugative plasmid, DNA finger printing showed a similar band pattern in plasmids. Conclusions: The dissemination of cephalosporin resistance was due to related plasmids carrying the ESBL genes. 2007 artículo científico 0036-3634 https://www.redalyc.org/articulo.oa?id=10649608 en http://www.redalyc.org/revista.oa?id=106 Salud Pública de México application/pdf Instituto Nacional de Salud Pública Salud Pública de México (México) Num.6 Vol.49