Human identification analysis using PCR from the root portion of dental elements under different conditions of temperature and exposure time
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| Format: | Artículo científico |
| Sprache: | en |
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Universidade da Região de Joinville
2012
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| _version_ | 1876435380537917440 |
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| author | Ricardo Henrique Alves da Silva |
| author_facet | Ricardo Henrique Alves da Silva |
| contents | Human identification analysis using PCR from the root portion of dental elements under different conditions of temperature and exposure time Ricardo Henrique Alves da Silva Rodrigo Quiezi Claudia Danielli Pereira Bertolacini Suzana Papile Maciel Carvalho Kellen Cristina da Silva Gasque Carina Thais de Almeida-e-Silva Lucilene Arilho Ribeiro Bicudo Medicina Forensic Sciences Forensic Dentistry Forensic Anthropology Introduction and objective: The main exogenous factors limiting the retrieval of information from human remains are fire and accidents involving high temperatures. Teeth, due to their relatively high degree of chemical and physical resistance, offer the possibility for the recovery of genetic material, becoming important in forensic cases. With the aim to contribute to a standardization of the protocols employed in DNA extraction and analysis, it was evaluated the integrity of DNA recovered from dental roots submitted to high temperatures, simulating what happens to burnt people. Material and methods: Extractions of genomic DNA were made from the dental root after exposure to high temperatures (600ºC, 800ºC and 1000ºC), during 10, 30 and 60 minutes. Results and conclusion: After molecular analysis through PCR technique, it was verified that DNA amplification of the samples was not possible at any of the periods and temperatures analyzed. 2012 artículo científico 1806-7727 https://www.redalyc.org/articulo.oa?id=153023690010 en http://www.redalyc.org/revista.oa?id=1530 RSBO Revista Sul-Brasileira de Odontologia application/pdf Universidade da Região de Joinville RSBO Revista Sul-Brasileira de Odontologia (Brasil) Num.1 Vol.9 |
| format | Artículo científico |
| id | redalyc_153023690010 |
| institution | Redalyc |
| language | en |
| publishDate | 2012 |
| publisher | Universidade da Região de Joinville |
| spellingShingle | Human identification analysis using PCR from the root portion of dental elements under different conditions of temperature and exposure time Ricardo Henrique Alves da Silva Medicina Forensic Sciences Forensic Dentistry Forensic Anthropology Human identification analysis using PCR from the root portion of dental elements under different conditions of temperature and exposure time Ricardo Henrique Alves da Silva Rodrigo Quiezi Claudia Danielli Pereira Bertolacini Suzana Papile Maciel Carvalho Kellen Cristina da Silva Gasque Carina Thais de Almeida-e-Silva Lucilene Arilho Ribeiro Bicudo Medicina Forensic Sciences Forensic Dentistry Forensic Anthropology Introduction and objective: The main exogenous factors limiting the retrieval of information from human remains are fire and accidents involving high temperatures. Teeth, due to their relatively high degree of chemical and physical resistance, offer the possibility for the recovery of genetic material, becoming important in forensic cases. With the aim to contribute to a standardization of the protocols employed in DNA extraction and analysis, it was evaluated the integrity of DNA recovered from dental roots submitted to high temperatures, simulating what happens to burnt people. Material and methods: Extractions of genomic DNA were made from the dental root after exposure to high temperatures (600ºC, 800ºC and 1000ºC), during 10, 30 and 60 minutes. Results and conclusion: After molecular analysis through PCR technique, it was verified that DNA amplification of the samples was not possible at any of the periods and temperatures analyzed. 2012 artículo científico 1806-7727 https://www.redalyc.org/articulo.oa?id=153023690010 en http://www.redalyc.org/revista.oa?id=1530 RSBO Revista Sul-Brasileira de Odontologia application/pdf Universidade da Região de Joinville RSBO Revista Sul-Brasileira de Odontologia (Brasil) Num.1 Vol.9 |
| title | Human identification analysis using PCR from the root portion of dental elements under different conditions of temperature and exposure time |
| topic | Medicina Forensic Sciences Forensic Dentistry Forensic Anthropology |
| url | https://www.redalyc.org/articulo.oa?id=153023690010 |