Solid-to-liquid extraction and HPLC/UV determination of amygdalin of seeds of apple (Malus pumila Mill): Comparison between traditional-solvent and microwave methodologies
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| Format: | Artículo científico |
| Language: | en |
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Universidad Nacional de Colombia
2018
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| _version_ | 1876451908197023744 |
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| author | Juan C. Amaya-Salcedo |
| author_facet | Juan C. Amaya-Salcedo |
| contents | Solid-to-liquid extraction and HPLC/UV determination of amygdalin of seeds of apple (Malus pumila Mill): Comparison between traditional-solvent and microwave methodologies Juan C. Amaya-Salcedo Oswaldo E. Cárdenas-González Jovanny A. Gómez-Castaño Agrociencias Laetrile Amygdalin Vitamin B17 Malus pumila Mill Cyanogenic glycosides This work evaluates the efficiency in the extraction of the cyanogenic glycoside amygdalin D-mandelonitrile- p-D-gentiobioside from seeds of apples (Malus pumila Mill) by using the two most conventional solid-to-liquid methodologies (reflux and Soxhlet) in relation to the more contemporary microwave technique. The extraction efficiencies are determined by an optimized and standardized high-performance liquid chromatography (HPLC) method that requires retention times of about 1 min only. The best chromatographic separation of amygdalin is found in extracts obtained using the reflux method; however, the highest total separation efficiency, requiring only 10% of total time and the solvents inverted in reflux or Soxhlet extraction, is reached by employing microwave radiation. In contrast, the higher extraction amounts of amygdalin from the seeds are obtained using the Soxhlet solid-to-liquid technique. With the Soxhlet methodology, quantities of amygdalin from apple seeds are obtained in excess of three times and twice those obtained with reflux and microwave methodologies, respectively. The purity and structural identification of the extracted amygdalin were verified spectroscopically using UV-vis, NMR and FTIR techniques. 2018 artículo científico 0120-2812 https://www.redalyc.org/articulo.oa?id=169959152001 https://www.redalyc.org/journal/1699/169959152001/ https://www.redalyc.org/journal/1699/169959152001/html/ https://www.redalyc.org/journal/1699/169959152001/169959152001.epub https://www.redalyc.org/journal/1699/169959152001/movil 10.15446/acag.v67n3.67186 en http://www.redalyc.org/revista.oa?id=1699 Acta Agronómica application/pdf Universidad Nacional de Colombia Acta Agronómica (Colombia) Num.3 Vol.67 |
| format | Artículo científico |
| id | redalyc_169959152001 |
| institution | Redalyc |
| language | en |
| publishDate | 2018 |
| publisher | Universidad Nacional de Colombia |
| spellingShingle | Solid-to-liquid extraction and HPLC/UV determination of amygdalin of seeds of apple (Malus pumila Mill): Comparison between traditional-solvent and microwave methodologies Juan C. Amaya-Salcedo Agrociencias Laetrile Amygdalin Vitamin B17 Malus pumila Mill Cyanogenic glycosides Solid-to-liquid extraction and HPLC/UV determination of amygdalin of seeds of apple (Malus pumila Mill): Comparison between traditional-solvent and microwave methodologies Juan C. Amaya-Salcedo Oswaldo E. Cárdenas-González Jovanny A. Gómez-Castaño Agrociencias Laetrile Amygdalin Vitamin B17 Malus pumila Mill Cyanogenic glycosides This work evaluates the efficiency in the extraction of the cyanogenic glycoside amygdalin D-mandelonitrile- p-D-gentiobioside from seeds of apples (Malus pumila Mill) by using the two most conventional solid-to-liquid methodologies (reflux and Soxhlet) in relation to the more contemporary microwave technique. The extraction efficiencies are determined by an optimized and standardized high-performance liquid chromatography (HPLC) method that requires retention times of about 1 min only. The best chromatographic separation of amygdalin is found in extracts obtained using the reflux method; however, the highest total separation efficiency, requiring only 10% of total time and the solvents inverted in reflux or Soxhlet extraction, is reached by employing microwave radiation. In contrast, the higher extraction amounts of amygdalin from the seeds are obtained using the Soxhlet solid-to-liquid technique. With the Soxhlet methodology, quantities of amygdalin from apple seeds are obtained in excess of three times and twice those obtained with reflux and microwave methodologies, respectively. The purity and structural identification of the extracted amygdalin were verified spectroscopically using UV-vis, NMR and FTIR techniques. 2018 artículo científico 0120-2812 https://www.redalyc.org/articulo.oa?id=169959152001 https://www.redalyc.org/journal/1699/169959152001/ https://www.redalyc.org/journal/1699/169959152001/html/ https://www.redalyc.org/journal/1699/169959152001/169959152001.epub https://www.redalyc.org/journal/1699/169959152001/movil 10.15446/acag.v67n3.67186 en http://www.redalyc.org/revista.oa?id=1699 Acta Agronómica application/pdf Universidad Nacional de Colombia Acta Agronómica (Colombia) Num.3 Vol.67 |
| title | Solid-to-liquid extraction and HPLC/UV determination of amygdalin of seeds of apple (Malus pumila Mill): Comparison between traditional-solvent and microwave methodologies |
| topic | Agrociencias Laetrile Amygdalin Vitamin B17 Malus pumila Mill Cyanogenic glycosides |
| url | https://www.redalyc.org/articulo.oa?id=169959152001 https://www.redalyc.org/journal/1699/169959152001/ https://www.redalyc.org/journal/1699/169959152001/html/ https://www.redalyc.org/journal/1699/169959152001/169959152001.epub https://www.redalyc.org/journal/1699/169959152001/movil |