Inmunohistoquímica en biopsias de piel tratadas con proteína K y microondas para el diagnóstico en animales persistentemente infectados con el virus diaria viral bovina
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| Formato: | Artículo científico |
| Lenguaje: | es |
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Universidad Austral de Chile
2003
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| _version_ | 1876477084359983104 |
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| author | J. LERTORA |
| author_facet | J. LERTORA |
| contents | Inmunohistoquímica en biopsias de piel tratadas con proteína K y microondas para el diagnóstico en animales persistentemente infectados con el virus diaria viral bovina J. LERTORA E. PAREDES G. REINHARDT A. ALBERDI Veterinaria skin biopsies Immunohistochemistry bovine viral diarrhea virus The aims of this study were to set up and to evaluate an immunohistochemical technique in bovine skin biopsies for the diagnosis of persistently infected cattle with Bovine Viral Diarrhea virus. Formalin fixed, paraffinembedded skin tissues were submitted to different treatments for antigen retrieval, incubated with 15.c.5, WB160, WB162, WB210 and WB215 monoclonal antibodies, and developed with the biotin-streptoavidin-peroxidase system. Monoclonal antibodies 15.c.5 and WB210, which react with the glycoprotein Erns of the Bovine Viral Diarrhea virus, successfully detected the virus after unmasking the antigen. The remaining monoclonal antibodies did not react in formalin fixed tissues. The stain pattern and its distribution were identical in all persistently infected bovines examined and is typical of this condition. The immunohistochemistry applied to formalinfixed skin biopsies using monoclonal antibody 15.c.5, previous enzymatic antigenic unmasking, is a useful method for the diagnosis of persistently infected bovines. 2003 artículo científico 0301-732X https://www.redalyc.org/articulo.oa?id=173013742003 es http://www.redalyc.org/revista.oa?id=1730 Archivos de Medicina Veterinaria application/pdf Universidad Austral de Chile Archivos de Medicina Veterinaria (Chile) Num.1 Vol.35 |
| format | Artículo científico |
| id | redalyc_173013742003 |
| institution | Redalyc |
| language | es |
| publishDate | 2003 |
| publisher | Universidad Austral de Chile |
| spellingShingle | Inmunohistoquímica en biopsias de piel tratadas con proteína K y microondas para el diagnóstico en animales persistentemente infectados con el virus diaria viral bovina J. LERTORA Veterinaria skin biopsies Immunohistochemistry bovine viral diarrhea virus Inmunohistoquímica en biopsias de piel tratadas con proteína K y microondas para el diagnóstico en animales persistentemente infectados con el virus diaria viral bovina J. LERTORA E. PAREDES G. REINHARDT A. ALBERDI Veterinaria skin biopsies Immunohistochemistry bovine viral diarrhea virus The aims of this study were to set up and to evaluate an immunohistochemical technique in bovine skin biopsies for the diagnosis of persistently infected cattle with Bovine Viral Diarrhea virus. Formalin fixed, paraffinembedded skin tissues were submitted to different treatments for antigen retrieval, incubated with 15.c.5, WB160, WB162, WB210 and WB215 monoclonal antibodies, and developed with the biotin-streptoavidin-peroxidase system. Monoclonal antibodies 15.c.5 and WB210, which react with the glycoprotein Erns of the Bovine Viral Diarrhea virus, successfully detected the virus after unmasking the antigen. The remaining monoclonal antibodies did not react in formalin fixed tissues. The stain pattern and its distribution were identical in all persistently infected bovines examined and is typical of this condition. The immunohistochemistry applied to formalinfixed skin biopsies using monoclonal antibody 15.c.5, previous enzymatic antigenic unmasking, is a useful method for the diagnosis of persistently infected bovines. 2003 artículo científico 0301-732X https://www.redalyc.org/articulo.oa?id=173013742003 es http://www.redalyc.org/revista.oa?id=1730 Archivos de Medicina Veterinaria application/pdf Universidad Austral de Chile Archivos de Medicina Veterinaria (Chile) Num.1 Vol.35 |
| title | Inmunohistoquímica en biopsias de piel tratadas con proteína K y microondas para el diagnóstico en animales persistentemente infectados con el virus diaria viral bovina |
| topic | Veterinaria skin biopsies Immunohistochemistry bovine viral diarrhea virus |
| url | https://www.redalyc.org/articulo.oa?id=173013742003 |