Ensayo rápido para la determinación simultánea de enrofloxacina y ciprofloxacina en leche mediante Cromatografía Líquida de Alto Rendimiento
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| Format: | Artículo científico |
| Sprache: | es |
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Universidad Austral de Chile
2001
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| _version_ | 1876457112450629632 |
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| author | A. San Martín |
| author_facet | A. San Martín |
| contents | Ensayo rápido para la determinación simultánea de enrofloxacina y ciprofloxacina en leche mediante Cromatografía Líquida de Alto Rendimiento A. San Martín H. Cañon D. Iragüen Veterinaria milk enrofloxacine ciprofloxacine Analytical methodology A fast liquid chromatography method with detection of fluorescence for enrofloxacine and ciprofloxacine residues in bovine milk (powder, pasteurized, raw) is reported. The procedures consisted of succesive extraction of milk with acidified ethanol concentrated by dryness and reconstituted for the chromatographic analysis. The analysis is performed by isocratic elution using an acetonitrile-2% acetic acid (15:85) mobile phase and silica dimetiloctadesil column with fluorescence detection at excitation and emission wavelengths of 278 and 450 nm. respectively. The limit of detection and quantification was 5 ng/ml for enrofloxacine and ciprofloxacine. Both analites presented a lineal behaviour when the different concentrations of the pure standars and contaminated matrixes were analyzed; the coefficients of correlation were higher than 0.99. The porcentages of recovery of both analites in the different matrixes were between 82.9 and 115%. 2001 artículo científico 0301-732X https://www.redalyc.org/articulo.oa?id=173013744014 es http://www.redalyc.org/revista.oa?id=1730 Archivos de Medicina Veterinaria application/pdf Universidad Austral de Chile Archivos de Medicina Veterinaria (Chile) Num.1 Vol.33 |
| format | Artículo científico |
| id | redalyc_173013744014 |
| institution | Redalyc |
| language | es |
| publishDate | 2001 |
| publisher | Universidad Austral de Chile |
| spellingShingle | Ensayo rápido para la determinación simultánea de enrofloxacina y ciprofloxacina en leche mediante Cromatografía Líquida de Alto Rendimiento A. San Martín Veterinaria milk enrofloxacine ciprofloxacine Analytical methodology Ensayo rápido para la determinación simultánea de enrofloxacina y ciprofloxacina en leche mediante Cromatografía Líquida de Alto Rendimiento A. San Martín H. Cañon D. Iragüen Veterinaria milk enrofloxacine ciprofloxacine Analytical methodology A fast liquid chromatography method with detection of fluorescence for enrofloxacine and ciprofloxacine residues in bovine milk (powder, pasteurized, raw) is reported. The procedures consisted of succesive extraction of milk with acidified ethanol concentrated by dryness and reconstituted for the chromatographic analysis. The analysis is performed by isocratic elution using an acetonitrile-2% acetic acid (15:85) mobile phase and silica dimetiloctadesil column with fluorescence detection at excitation and emission wavelengths of 278 and 450 nm. respectively. The limit of detection and quantification was 5 ng/ml for enrofloxacine and ciprofloxacine. Both analites presented a lineal behaviour when the different concentrations of the pure standars and contaminated matrixes were analyzed; the coefficients of correlation were higher than 0.99. The porcentages of recovery of both analites in the different matrixes were between 82.9 and 115%. 2001 artículo científico 0301-732X https://www.redalyc.org/articulo.oa?id=173013744014 es http://www.redalyc.org/revista.oa?id=1730 Archivos de Medicina Veterinaria application/pdf Universidad Austral de Chile Archivos de Medicina Veterinaria (Chile) Num.1 Vol.33 |
| title | Ensayo rápido para la determinación simultánea de enrofloxacina y ciprofloxacina en leche mediante Cromatografía Líquida de Alto Rendimiento |
| topic | Veterinaria milk enrofloxacine ciprofloxacine Analytical methodology |
| url | https://www.redalyc.org/articulo.oa?id=173013744014 |