Uso de bacteriófagos en gallinas de postura infectadas con Salmonella enterica serotipo Enteritidis: prevención de la colonización intestinal y reproductiva
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| Format: | Artículo científico |
| Language: | es |
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Universidad Austral de Chile
2011
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| author | C Borie |
| author_facet | C Borie |
| contents | Uso de bacteriófagos en gallinas de postura infectadas con Salmonella enterica serotipo Enteritidis: prevención de la colonización intestinal y reproductiva C Borie C Hauva J Quiroga V Bravo ML Sánchez MA Morales P Retamal J Retamales J Robeson Veterinaria poultry Salmonella prevention laying hens bacteriophages Salmonella enterica serovar Enteritidis (SE) remains an important enteropathogen in the poultry industry and public health. Due to the limited effectiveness of control measures, lytic bacteriophages have shown a potential use as biocontrollers of SE in birds. The aim of this study was to determine the effectiveness of prophylactic therapy with phages to control intestinal and reproductive tract colonization of SE in laying hens. 22-week old Hy-Line Brown hens free of Salmonella, were treated with a mixture of three bacteriophages (10(11) PFU/dose/phage) and challenged with 2.4 x 10(8) CFU of SE, 24 hours post phage treatment. On day 10 post challenge, hens were euthanatized, and individual samples of cecum, ovary and oviduct, were analyzed using qualitative and quantitative bacteriology. Eggs laid during the experience were collected and processed to detect SE. The incidence of Salmonella in ceca was similar between positive control and treated groups (96.67%) and cecal bacterial counts did not present significant differences between them (P > 0.05). In reproductive tissues, phagetherapy was able to slightly reduce the SE count in the ovary (P < 0.05), but not in oviducts (P > 0.05). This lytic activity of phages observed in ovaric tissue, encourages further efforts to elucidate the real contribution of bacteriophages as SE biocontrollers in laying hens. 2011 artículo científico 0301-732X https://www.redalyc.org/articulo.oa?id=173019392012 es http://www.redalyc.org/revista.oa?id=1730 Archivos de Medicina Veterinaria application/pdf Universidad Austral de Chile Archivos de Medicina Veterinaria (Chile) Num.1 Vol.43 |
| format | Artículo científico |
| id | redalyc_173019392012 |
| institution | Redalyc |
| language | es |
| publishDate | 2011 |
| publisher | Universidad Austral de Chile |
| spellingShingle | Uso de bacteriófagos en gallinas de postura infectadas con Salmonella enterica serotipo Enteritidis: prevención de la colonización intestinal y reproductiva C Borie Veterinaria poultry Salmonella prevention laying hens bacteriophages Uso de bacteriófagos en gallinas de postura infectadas con Salmonella enterica serotipo Enteritidis: prevención de la colonización intestinal y reproductiva C Borie C Hauva J Quiroga V Bravo ML Sánchez MA Morales P Retamal J Retamales J Robeson Veterinaria poultry Salmonella prevention laying hens bacteriophages Salmonella enterica serovar Enteritidis (SE) remains an important enteropathogen in the poultry industry and public health. Due to the limited effectiveness of control measures, lytic bacteriophages have shown a potential use as biocontrollers of SE in birds. The aim of this study was to determine the effectiveness of prophylactic therapy with phages to control intestinal and reproductive tract colonization of SE in laying hens. 22-week old Hy-Line Brown hens free of Salmonella, were treated with a mixture of three bacteriophages (10(11) PFU/dose/phage) and challenged with 2.4 x 10(8) CFU of SE, 24 hours post phage treatment. On day 10 post challenge, hens were euthanatized, and individual samples of cecum, ovary and oviduct, were analyzed using qualitative and quantitative bacteriology. Eggs laid during the experience were collected and processed to detect SE. The incidence of Salmonella in ceca was similar between positive control and treated groups (96.67%) and cecal bacterial counts did not present significant differences between them (P > 0.05). In reproductive tissues, phagetherapy was able to slightly reduce the SE count in the ovary (P < 0.05), but not in oviducts (P > 0.05). This lytic activity of phages observed in ovaric tissue, encourages further efforts to elucidate the real contribution of bacteriophages as SE biocontrollers in laying hens. 2011 artículo científico 0301-732X https://www.redalyc.org/articulo.oa?id=173019392012 es http://www.redalyc.org/revista.oa?id=1730 Archivos de Medicina Veterinaria application/pdf Universidad Austral de Chile Archivos de Medicina Veterinaria (Chile) Num.1 Vol.43 |
| title | Uso de bacteriófagos en gallinas de postura infectadas con Salmonella enterica serotipo Enteritidis: prevención de la colonización intestinal y reproductiva |
| topic | Veterinaria poultry Salmonella prevention laying hens bacteriophages |
| url | https://www.redalyc.org/articulo.oa?id=173019392012 |