Loop-mediated isothermal amplification assay for the detection of Ehrlichia canis DNA in blood samples from dogs
Fuente:
Redalyc
Salvato in:
| Autore principale: | |
|---|---|
| Natura: | Artículo científico |
| Lingua: | en |
| Pubblicazione: |
Universidad Austral de Chile
2013
|
| Soggetti: | |
| Accesso online: | |
| Tags: |
Aggiungi Tag
Nessun Tag, puoi essere il primo ad aggiungerne!!
|
| _version_ | 1876441732836491264 |
|---|---|
| author | SA Faggion |
| author_facet | SA Faggion |
| contents | Loop-mediated isothermal amplification assay for the detection of Ehrlichia canis DNA in blood samples from dogs SA Faggion AR Salvador KL Jacobino LFB Bortolotto MB Lopes M Silva EV Santos AL Fachin SC França M Marins Veterinaria LAMP Ehrlichia canis Canine monocytic ehrlichiosis The rickettsial bacterium Ehrlichia canis is the etiological agent of canine monocytic ehrlichiosis, one of the most important canine tick-borne diseases in the world. In this study, a loop-mediated isothermal amplification (LAMP) assay was developed for detection of E. canis DNA using LAMP primers targeting the groESL operon. Reactions were performed at 60°C for 60 min and the results were visualized by gel electrophoresis. Successful amplification was obtained using plasmid DNA containing a fragment of the groESL operon and DNA extracted from blood samples that tested positive for E. canis by real-time PCR. The specificity of amplification was confirmed by EcoRI restriction of internal sites in the LAMP primers and no cross- reactivity with blood samples positive for Babesia spp., another common tick-borne pathogen, was observed. The high cost of nucleic acid tests (NAT) is one of the disadvantages for their large-scale use as routine diagnostic tests. The E. canis LAMP assay developed here is an interesting alternative to PCR since it does not require a thermocycler, thus reducing costs for the veterinary clinical laboratory. 2013 artículo científico 0301-732X https://www.redalyc.org/articulo.oa?id=173028392012 en http://www.redalyc.org/revista.oa?id=1730 Archivos de Medicina Veterinaria application/pdf Universidad Austral de Chile Archivos de Medicina Veterinaria (Chile) Num.2 Vol.45 |
| format | Artículo científico |
| id | redalyc_173028392012 |
| institution | Redalyc |
| language | en |
| publishDate | 2013 |
| publisher | Universidad Austral de Chile |
| spellingShingle | Loop-mediated isothermal amplification assay for the detection of Ehrlichia canis DNA in blood samples from dogs SA Faggion Veterinaria LAMP Ehrlichia canis Canine monocytic ehrlichiosis Loop-mediated isothermal amplification assay for the detection of Ehrlichia canis DNA in blood samples from dogs SA Faggion AR Salvador KL Jacobino LFB Bortolotto MB Lopes M Silva EV Santos AL Fachin SC França M Marins Veterinaria LAMP Ehrlichia canis Canine monocytic ehrlichiosis The rickettsial bacterium Ehrlichia canis is the etiological agent of canine monocytic ehrlichiosis, one of the most important canine tick-borne diseases in the world. In this study, a loop-mediated isothermal amplification (LAMP) assay was developed for detection of E. canis DNA using LAMP primers targeting the groESL operon. Reactions were performed at 60°C for 60 min and the results were visualized by gel electrophoresis. Successful amplification was obtained using plasmid DNA containing a fragment of the groESL operon and DNA extracted from blood samples that tested positive for E. canis by real-time PCR. The specificity of amplification was confirmed by EcoRI restriction of internal sites in the LAMP primers and no cross- reactivity with blood samples positive for Babesia spp., another common tick-borne pathogen, was observed. The high cost of nucleic acid tests (NAT) is one of the disadvantages for their large-scale use as routine diagnostic tests. The E. canis LAMP assay developed here is an interesting alternative to PCR since it does not require a thermocycler, thus reducing costs for the veterinary clinical laboratory. 2013 artículo científico 0301-732X https://www.redalyc.org/articulo.oa?id=173028392012 en http://www.redalyc.org/revista.oa?id=1730 Archivos de Medicina Veterinaria application/pdf Universidad Austral de Chile Archivos de Medicina Veterinaria (Chile) Num.2 Vol.45 |
| title | Loop-mediated isothermal amplification assay for the detection of Ehrlichia canis DNA in blood samples from dogs |
| topic | Veterinaria LAMP Ehrlichia canis Canine monocytic ehrlichiosis |
| url | https://www.redalyc.org/articulo.oa?id=173028392012 |