Identification of Nosema ceranae in the Valparaíso District, Chile
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| Format: | Artículo científico |
| Sprache: | en |
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Universidad Austral de Chile
2014
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| _version_ | 1876444936119779328 |
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| author | J Bravo |
| author_facet | J Bravo |
| contents | Identification of Nosema ceranae in the Valparaíso District, Chile J Bravo V Carbonell JT Valdebenito C Figueroa CE Valdovinos R Martín-Hernández M Higes C Delporte Veterinaria Nosemosis Nosema apis Nosema ceranae Apis mellifera This study aimed to demonstrate for the first time the presence of Nosema ceranae in the Valparaíso District. The analised bee samples were obtained in the Valparaíso Region, between 2011 and 2012, from beehives located in San Antonio, Limache and La Ligua. Samples obtained from groups of 60 bees were macerated and analysed by phase contrast microscopy at 40x. Then, DNA was extracted and the gene that encodes for 16SrRNA of Nosema apis and N. ceranae was amplified by multiplex PCR. 100% of the samples were infected by N. ceranae , while N. apis was not detected. During the period of study 2,915 colonies died in the Valparaíso Region which is 47.3% of the total hives of the Region. The loss of beehives during this period in the V Region of Chile could be related to either the lack of training in management and health monitoring of the colonies or the presence of N. ceranae. 2014 artículo científico 0301-732X https://www.redalyc.org/articulo.oa?id=173033278021 en http://www.redalyc.org/revista.oa?id=1730 Archivos de Medicina Veterinaria application/pdf Universidad Austral de Chile Archivos de Medicina Veterinaria (Chile) Num.3 Vol.46 |
| format | Artículo científico |
| id | redalyc_173033278021 |
| institution | Redalyc |
| language | en |
| publishDate | 2014 |
| publisher | Universidad Austral de Chile |
| spellingShingle | Identification of Nosema ceranae in the Valparaíso District, Chile J Bravo Veterinaria Nosemosis Nosema apis Nosema ceranae Apis mellifera Identification of Nosema ceranae in the Valparaíso District, Chile J Bravo V Carbonell JT Valdebenito C Figueroa CE Valdovinos R Martín-Hernández M Higes C Delporte Veterinaria Nosemosis Nosema apis Nosema ceranae Apis mellifera This study aimed to demonstrate for the first time the presence of Nosema ceranae in the Valparaíso District. The analised bee samples were obtained in the Valparaíso Region, between 2011 and 2012, from beehives located in San Antonio, Limache and La Ligua. Samples obtained from groups of 60 bees were macerated and analysed by phase contrast microscopy at 40x. Then, DNA was extracted and the gene that encodes for 16SrRNA of Nosema apis and N. ceranae was amplified by multiplex PCR. 100% of the samples were infected by N. ceranae , while N. apis was not detected. During the period of study 2,915 colonies died in the Valparaíso Region which is 47.3% of the total hives of the Region. The loss of beehives during this period in the V Region of Chile could be related to either the lack of training in management and health monitoring of the colonies or the presence of N. ceranae. 2014 artículo científico 0301-732X https://www.redalyc.org/articulo.oa?id=173033278021 en http://www.redalyc.org/revista.oa?id=1730 Archivos de Medicina Veterinaria application/pdf Universidad Austral de Chile Archivos de Medicina Veterinaria (Chile) Num.3 Vol.46 |
| title | Identification of Nosema ceranae in the Valparaíso District, Chile |
| topic | Veterinaria Nosemosis Nosema apis Nosema ceranae Apis mellifera |
| url | https://www.redalyc.org/articulo.oa?id=173033278021 |