Identification of contaminating bacteria when attempting to isolate Mycobacterium avium subsp. paratuberculosis (MAP) from bovine faecal and tissue samples using the BACTEC MGIT 960 system
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| Format: | Artículo científico |
| Langue: | en |
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Universidad Austral de Chile
2015
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| _version_ | 1876471757003554816 |
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| author | P Steuer |
| author_facet | P Steuer |
| contents | Identification of contaminating bacteria when attempting to isolate Mycobacterium avium subsp. paratuberculosis (MAP) from bovine faecal and tissue samples using the BACTEC MGIT 960 system P Steuer J de Waard MT Collins EP Troncoso OA Martínez C Tejeda MA Salgado Veterinaria PRA RISA hps 65 cultivo líquido paratuberculosis Diagnosis of Mycobacterium avium subsp. paratuberculosis infection by liquid culture is sensitive, faster than conventional solid culture and automated. However, a disadvantage of these culture systems is the potential for high frequency of culture contamination. Contaminant bacteria were identified as a step toward better contaminant control. No mycobacteria were detected by mycobacterial Polymerase Chain Reaction-Restriction Enzyme Analysis (PRA)- hsp 65. Ribosomal Intergenic Spacer Analysis (RISA) followed by sequence analysis identified Paenibacillus sp., Enterobacteriaceae and Pseudomonas aeruginosa as common contaminants. The present study aimed to identify a representative sample of contaminants encountered when culturing clinical faecal samples from Chilean cattle. Further studies involving a larger and more representative sample of animals are required to extrapolate the results to a broader population. 2015 artículo científico 0301-732X https://www.redalyc.org/articulo.oa?id=173041601015 en http://www.redalyc.org/revista.oa?id=1730 Archivos de Medicina Veterinaria application/pdf Universidad Austral de Chile Archivos de Medicina Veterinaria (Chile) Num.1 Vol.47 |
| format | Artículo científico |
| id | redalyc_173041601015 |
| institution | Redalyc |
| language | en |
| publishDate | 2015 |
| publisher | Universidad Austral de Chile |
| spellingShingle | Identification of contaminating bacteria when attempting to isolate Mycobacterium avium subsp. paratuberculosis (MAP) from bovine faecal and tissue samples using the BACTEC MGIT 960 system P Steuer Veterinaria PRA RISA hps 65 cultivo líquido paratuberculosis Identification of contaminating bacteria when attempting to isolate Mycobacterium avium subsp. paratuberculosis (MAP) from bovine faecal and tissue samples using the BACTEC MGIT 960 system P Steuer J de Waard MT Collins EP Troncoso OA Martínez C Tejeda MA Salgado Veterinaria PRA RISA hps 65 cultivo líquido paratuberculosis Diagnosis of Mycobacterium avium subsp. paratuberculosis infection by liquid culture is sensitive, faster than conventional solid culture and automated. However, a disadvantage of these culture systems is the potential for high frequency of culture contamination. Contaminant bacteria were identified as a step toward better contaminant control. No mycobacteria were detected by mycobacterial Polymerase Chain Reaction-Restriction Enzyme Analysis (PRA)- hsp 65. Ribosomal Intergenic Spacer Analysis (RISA) followed by sequence analysis identified Paenibacillus sp., Enterobacteriaceae and Pseudomonas aeruginosa as common contaminants. The present study aimed to identify a representative sample of contaminants encountered when culturing clinical faecal samples from Chilean cattle. Further studies involving a larger and more representative sample of animals are required to extrapolate the results to a broader population. 2015 artículo científico 0301-732X https://www.redalyc.org/articulo.oa?id=173041601015 en http://www.redalyc.org/revista.oa?id=1730 Archivos de Medicina Veterinaria application/pdf Universidad Austral de Chile Archivos de Medicina Veterinaria (Chile) Num.1 Vol.47 |
| title | Identification of contaminating bacteria when attempting to isolate Mycobacterium avium subsp. paratuberculosis (MAP) from bovine faecal and tissue samples using the BACTEC MGIT 960 system |
| topic | Veterinaria PRA RISA hps 65 cultivo líquido paratuberculosis |
| url | https://www.redalyc.org/articulo.oa?id=173041601015 |