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Main Author:	Mirtha Latsague
Format:	Artículo científico
Language:	es
Published:	Universidad Austral de Chile 2007
Subjects:	Agrociencias Nothofagus AIA oxidasa enraizamiento ácido indol acético
Online Access:	https://www.redalyc.org/articulo.oa?id=173113288006
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Actividad de AIA oxidasa en estacas de especies del género Nothofagus Mirtha Latsague Patricia Sáez Marco Paredes Edith Alarcón Agrociencias Nothofagus AIA oxidasa enraizamiento ácido indol acético Entre las distintas especies de Nothofagus existe variación en la capacidad de enraizamiento. Las estacas de lenga (N. pumilio) presentan una respuesta casi nula al enraizamiento, comparada con raulí (N. alpina) y roble (N. obliqua). Según antecedentes recopilados en literatura, los niveles endógenos de ácido indol acético (AIA), hormona responsable de la formación de raíces adventicias, varían según los niveles de actividad de AIA oxidasa (AIA-O). Basados en estas observaciones, la actividad de AIA-O fue comparativamente analizada en estacas de estas tres especies de Nothofagus. Para cuantificar la actividad enzimática se realizó un ensayo de espectrofotometría, midiendo a 535 nm λ. La cantidad residual de AIA se estimó por referencia a una curva de calibración. La actividad enzimática fue expresada en mM de AIA mg de proteína-1 h-1. Los resultados muestran que N. pumilio presenta una mayor actividad enzimática de AIA oxidasa en comparación con las otras especies analizadas (P < 0,05). Estos resultados respaldarían, al menos parcialmente, las diferencias en la capacidad de rizogénesis en las especies de Nothofagus estudiadas. 2007 artículo científico 0304-8799 https://www.redalyc.org/articulo.oa?id=173113288006 es http://www.redalyc.org/revista.oa?id=1731 Bosque application/pdf Universidad Austral de Chile Bosque (Chile) Num.2 Vol.28

if (filemtime($filename) + $maxlifetime < time()) { unlink($filename); $count++; } } return $count; } /** * A function that is called internally when session data is to be saved. * * @param string $sessId The current session ID * @param string $data The session data to write * * @return bool */ protected function saveSession($sessId, $data): bool { $sessFile = $this->path . '/sess_' . $sessId; if ($handle = fopen($sessFile, 'w')) { $return = false; // Lock the file for exclusive access to avoid issues with multiple // processes writing session simultaneously: if (flock($handle, LOCK_EX)) { $return = fwrite($handle, $data); // Make sure that there's no trailing data by truncating the file to // the correct length: ftruncate($handle, strlen($data)); fflush($handle); flock($handle, LOCK_UN); } fclose($handle); if ($return !== false) { return true; } } // If we got this far, something went wrong with the file output... // It is tempting to throw an exception here, but this code is called // outside of the context of exception handling, so all we can do is // echo a message.

// Anecdotal testing Today and Yesterday seems to indicate destroy() // is called by the garbage collector and everything is good. // Something to keep in mind though. return 0; } /** * Write function that is called when session data is to be saved. * * @param string $sessId The current session ID * @param string $data The session data to write * * @return bool */ public function write($sessId, $data): bool { if ($this->writesDisabled) { return true; } return $this->saveSession($sessId, $data); } /** * A function that is called internally when session data is to be saved. * * @param string $sessId The current session ID * @param string $data The session data to write * * @return bool */ abstract protected function saveSession($sessId, $data): bool; }

* * @return void */ public function writeClose() { // The assumption is that we're using PHP's ext/session. // session_write_close() will actually overwrite $_SESSION with an // empty array on completion -- which leads to a mismatch between what // is in the storage object and $_SESSION. To get around this, we // temporarily reset $_SESSION to an array, and then re-link it to // the storage object. // // Additionally, while you _can_ write to $_SESSION following a // session_write_close() operation, no changes made to it will be // flushed to the session handler. As such, we now mark the storage // object isImmutable. $storage = $this->getStorage(); if (! $storage->isImmutable()) { $_SESSION = $storage->toArray(true); session_write_close(); $storage->fromArray($_SESSION); $storage->markImmutable(); } } /** * Attempt to set the session name * * If the session has already been started, or if the name provided fails * validation, an exception will be raised. * * @param string $name * @return SessionManager * @throws Exception\InvalidArgumentException */ public function setName($name) { if ($this->sessionExists()) { throw new Exception\InvalidArgumentException( 'Cannot set session name after a session has already started'

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