Saved in:
| Main Author: | |
|---|---|
| Format: | Artículo científico |
| Language: | en |
| Published: |
Fundação APINCO de Ciência e Tecnologia Avícolas
2015
|
| Subjects: | |
| Online Access: | https://www.redalyc.org/articulo.oa?id=179742203003 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
Table of Contents:
- Determining the Best Sectioning Method and Intestinal Segment for Morphometric Analysis in Broilers MS Gava LB Moraes D Carvalho GZ Chitolina LCB Fallavena HLS Moraes J Herpich CTP Salle Agrociencias Image villus poultry intestine morphometry Brazilian poultry production is very efficient and demands maximum broiler performance. Therefore, digestive system pathologies have a relevant role. Considering it is difficult to obtain consistent information on intestinal morphometric analysis, this study aimed at establishing essential and clear criteria for the collection of intestinal segments for morphometric analysis. Fifteen 13-d-old broilers were sacrificed and three intestinal segments were collected per bird. Two 3-cm long sections were obtained from each of the intestinal segments. Samples were collected open or closed. The closed samples were transversely, hemicylindrically, or longitudinally sectioned. Samples were processed and stained with hematoxylin and eosin. The number of microscopic fields in each section was counted. Villi presenting the base clearly embedded in the submucosa, no damage or folds, and simple columnar epithelium at the tip were considered adequate for measurements. These villi were counted in each sample. The results shows that hemicylindrical sections presented the highest number of observation fields, with an average of 9.76 fields. Jejunum samples were among the three highest average villi counts, with 18.23 in longitudinal sections and 15.61 in hemicylindrical sections. The results of the present study indicate that hemicylindrical sectioning and jejunal samples were, respectively, the best sectioning method and the best intestinal segment for the morphometric analysis of the intestines of broilers. 2015 artículo científico 1516-635X https://www.redalyc.org/articulo.oa?id=179742203003 en http://www.redalyc.org/revista.oa?id=1797 Revista Brasileira de Ciência Avícola application/pdf Fundação APINCO de Ciência e Tecnologia Avícolas Revista Brasileira de Ciência Avícola (Brasil) Num.2 Vol.17