In-Vitro Adhesion and Invasion Properties of Salmonella Typhimurium Competing with Bacteriophage in Epithelial Cells and Chicken Macrophages
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| Formato: | Artículo científico |
| Lenguaje: | en |
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Fundação APINCO de Ciência e Tecnologia Avícolas
2015
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| _version_ | 1876491341230243840 |
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| author | HY Lee |
| author_facet | HY Lee |
| contents | In-Vitro Adhesion and Invasion Properties of Salmonella Typhimurium Competing with Bacteriophage in Epithelial Cells and Chicken Macrophages HY Lee D Biswas J Ahn Agrociencias Toll Adhesion Invasion Salmonella Bacteriophage This study was designed to assess the role of bacteriophage P22 in the adhesion, invasion, intracellular survival of, and cellular immune response to Salmonella Typhimurium in intestinal epithelial INT-407 and chicken macrophage-like HD11 cells. The ability of S. Typhimurium to adhere, invade, and survive to INT-407 and HD11cells was evaluated under Salmonella infection alone (control), phage treatment followed by Salmonella infection (PS), Salmonella infection followed by phage treatment (SP), and a combination treatment with Salmonella and phage (S+P). The number of S. Typhimurium associated on INT-407 cells was reduced from 4.2 to 2.7 log cfu/cm2 by phage treatment (SP). The number of intracellular S. Typhimurium within INT-407 cells was significantly reduced to below the detection limit (0.7 log cfu/cm2) compared with the control (3.4 log cfu/cm2). S. Typhimurium remained inside HD11 cells at 49% and 17% levels in the absence and presence of phages, respectively, at 24 h post-infection (hpi). The expression levels of IFN-g, IL-10, IL-1b, IL-6, IL-8, iNOS, and IL-12 increased in HD11 cells regardless the absence and presence of phages, while those of IL-16, TLR2-1, TLR3, and TLR7 were decreased at 0 and 24 hpi. This study sheds new light on our understanding of the role of phages in Salmonella adhesion, invasion, survival, and cellular immune responses. 2015 artículo científico 1516-635X https://www.redalyc.org/articulo.oa?id=179743242003 en http://www.redalyc.org/revista.oa?id=1797 Revista Brasileira de Ciência Avícola application/pdf Fundação APINCO de Ciência e Tecnologia Avícolas Revista Brasileira de Ciência Avícola (Brasil) Num.4 Vol.17 |
| format | Artículo científico |
| id | redalyc_179743242003 |
| institution | Redalyc |
| language | en |
| publishDate | 2015 |
| publisher | Fundação APINCO de Ciência e Tecnologia Avícolas |
| spellingShingle | In-Vitro Adhesion and Invasion Properties of Salmonella Typhimurium Competing with Bacteriophage in Epithelial Cells and Chicken Macrophages HY Lee Agrociencias Toll Adhesion Invasion Salmonella Bacteriophage In-Vitro Adhesion and Invasion Properties of Salmonella Typhimurium Competing with Bacteriophage in Epithelial Cells and Chicken Macrophages HY Lee D Biswas J Ahn Agrociencias Toll Adhesion Invasion Salmonella Bacteriophage This study was designed to assess the role of bacteriophage P22 in the adhesion, invasion, intracellular survival of, and cellular immune response to Salmonella Typhimurium in intestinal epithelial INT-407 and chicken macrophage-like HD11 cells. The ability of S. Typhimurium to adhere, invade, and survive to INT-407 and HD11cells was evaluated under Salmonella infection alone (control), phage treatment followed by Salmonella infection (PS), Salmonella infection followed by phage treatment (SP), and a combination treatment with Salmonella and phage (S+P). The number of S. Typhimurium associated on INT-407 cells was reduced from 4.2 to 2.7 log cfu/cm2 by phage treatment (SP). The number of intracellular S. Typhimurium within INT-407 cells was significantly reduced to below the detection limit (0.7 log cfu/cm2) compared with the control (3.4 log cfu/cm2). S. Typhimurium remained inside HD11 cells at 49% and 17% levels in the absence and presence of phages, respectively, at 24 h post-infection (hpi). The expression levels of IFN-g, IL-10, IL-1b, IL-6, IL-8, iNOS, and IL-12 increased in HD11 cells regardless the absence and presence of phages, while those of IL-16, TLR2-1, TLR3, and TLR7 were decreased at 0 and 24 hpi. This study sheds new light on our understanding of the role of phages in Salmonella adhesion, invasion, survival, and cellular immune responses. 2015 artículo científico 1516-635X https://www.redalyc.org/articulo.oa?id=179743242003 en http://www.redalyc.org/revista.oa?id=1797 Revista Brasileira de Ciência Avícola application/pdf Fundação APINCO de Ciência e Tecnologia Avícolas Revista Brasileira de Ciência Avícola (Brasil) Num.4 Vol.17 |
| title | In-Vitro Adhesion and Invasion Properties of Salmonella Typhimurium Competing with Bacteriophage in Epithelial Cells and Chicken Macrophages |
| topic | Agrociencias Toll Adhesion Invasion Salmonella Bacteriophage |
| url | https://www.redalyc.org/articulo.oa?id=179743242003 |