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| Natura: | Artículo científico |
| Lingua: | en |
| Pubblicazione: |
Fundação APINCO de Ciência e Tecnologia Avícolas
2015
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| Accesso online: | https://www.redalyc.org/articulo.oa?id=179743242013 |
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Sommario:
- Single Nucleotide Polymorphisms in IGFBP-2 Gene and Their Associations with Body Weight Traits on Jinghai Yellow Chicken XH Zhao MY Li SS Xu GJ Liu Agrociencias IGFBP 2 gene PCRSSCP Body weight Polymorphism Insulin-like growth factor binding protein-2 (IGFBP-2) regulates a broad spectrum of biological activities involved in growth, development, and differentiation. This study aimed at comparing polymorphisms in intron2 of the IGFBP-2 gene among four chicken breeds and at analyzing the associations between its genotypes and body weight in Jinghai Yellow chicken by using PCR-SSCP technique. For primer P2, three genotypes (AA, AB and BB) were observed in the four chicken breeds. Gene sequencing revealed one insertion/deletion (the inserted/ deleted TC after position 552bp) in the intron 2 of IGFBP-2 gene. For primer P5, three genotypes were identified in Jinghai Yellow chickens, and named CC, CD and DD. Gene sequencing revealed two SNPs (C1107G, C1130T) and one inserted/deleted GCCAGGT after 1115bp in the intron 2 of IGFBP-2 gene. The results of the linear model analysis showed that Jinghai Yellow chickens with AA genotype had significantly heavier body weight, at hatch and 12 weeks of age, than those of the AB genotype (p<0.05). The A allele had a positive effect on body weight. We speculate that mutations in intron 2 could be used as genetic markers for body weight in Jinghai Yellow chicken. This study provides valuable information for the protection of genetic resources and for breeding of Jinghai Yellow chicken. 2015 artículo científico 1516-635X https://www.redalyc.org/articulo.oa?id=179743242013 en http://www.redalyc.org/revista.oa?id=1797 Revista Brasileira de Ciência Avícola application/pdf Fundação APINCO de Ciência e Tecnologia Avícolas Revista Brasileira de Ciência Avícola (Brasil) Num.4 Vol.17