Investigation of Influenza A, West Nile and Newcastle Disease Viruses in Birds from the Pantanal Wetlands of Mato Grosso, Brazil
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| Natura: | Artículo científico |
| Lingua: | en |
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Fundação APINCO de Ciência e Tecnologia Avícolas
2016
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| _version_ | 1876449057068548096 |
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| author | LB Pinto |
| author_facet | LB Pinto |
| contents | Investigation of Influenza A, West Nile and Newcastle Disease Viruses in Birds from the Pantanal Wetlands of Mato Grosso, Brazil LB Pinto T Ometto J Araújo LM Thomazelli MM Seixas CM Barbosa DGS Ramos ALT Melo JB Pinho EL Durigon DM Aguiar Agrociencias RT PCR qRT Virus Pantanal birds The Pantanal is the world’s largest wetland biome with a seasonal flood pulse that attracts a great diversity of birds, many of which are migratory. Birds can be natural reservoirs Influenza A , West Nile and Newcastle Disease viruses. However, the occurrence of carriers for these viruses in the Pantanal was not verified yet. The present study evaluated the occurrence of natural infection by Influenza A, WN and ND virus of birds in the municipality of Poconé, a subregion of the Pantanal in the state of Mato Grosso, Brazil. A total of 76 birds belonging to 11 orders and 20 families were captured using mist nets. The most representative order was Passeriformes, followed by the other nine orders, which included Columbiformes, Psittaciformes, Charadriiformes and Anseriformes. The most representative family was Thamnophilidae, with 16 individuals (21.0%), followed by the family Tyrannidae with 10 individuals (7.6%) and the family Furnariidae, with eight individuals (10.5%). The bird species were identified, and cloacal and tracheal swab samples were collected. The samples were subjected to RNA extraction and tested for the presence of the three agents by real-time polymerase chain reaction (qRT-PCR). All the sampled birds were considered healthy, had no clinical sign of infection, and were tested negative for the three viruses. Based on our findings, we can conclude that Influenza , West Nile and Newcastle Disease viruses were absent from the sample. 2016 artículo científico 1516-635X https://www.redalyc.org/articulo.oa?id=179746750013 en http://www.redalyc.org/revista.oa?id=1797 Revista Brasileira de Ciência Avícola application/pdf Fundação APINCO de Ciência e Tecnologia Avícolas Revista Brasileira de Ciência Avícola (Brasil) Num.2 Vol.18 |
| format | Artículo científico |
| id | redalyc_179746750013 |
| institution | Redalyc |
| language | en |
| publishDate | 2016 |
| publisher | Fundação APINCO de Ciência e Tecnologia Avícolas |
| spellingShingle | Investigation of Influenza A, West Nile and Newcastle Disease Viruses in Birds from the Pantanal Wetlands of Mato Grosso, Brazil LB Pinto Agrociencias RT PCR qRT Virus Pantanal birds Investigation of Influenza A, West Nile and Newcastle Disease Viruses in Birds from the Pantanal Wetlands of Mato Grosso, Brazil LB Pinto T Ometto J Araújo LM Thomazelli MM Seixas CM Barbosa DGS Ramos ALT Melo JB Pinho EL Durigon DM Aguiar Agrociencias RT PCR qRT Virus Pantanal birds The Pantanal is the world’s largest wetland biome with a seasonal flood pulse that attracts a great diversity of birds, many of which are migratory. Birds can be natural reservoirs Influenza A , West Nile and Newcastle Disease viruses. However, the occurrence of carriers for these viruses in the Pantanal was not verified yet. The present study evaluated the occurrence of natural infection by Influenza A, WN and ND virus of birds in the municipality of Poconé, a subregion of the Pantanal in the state of Mato Grosso, Brazil. A total of 76 birds belonging to 11 orders and 20 families were captured using mist nets. The most representative order was Passeriformes, followed by the other nine orders, which included Columbiformes, Psittaciformes, Charadriiformes and Anseriformes. The most representative family was Thamnophilidae, with 16 individuals (21.0%), followed by the family Tyrannidae with 10 individuals (7.6%) and the family Furnariidae, with eight individuals (10.5%). The bird species were identified, and cloacal and tracheal swab samples were collected. The samples were subjected to RNA extraction and tested for the presence of the three agents by real-time polymerase chain reaction (qRT-PCR). All the sampled birds were considered healthy, had no clinical sign of infection, and were tested negative for the three viruses. Based on our findings, we can conclude that Influenza , West Nile and Newcastle Disease viruses were absent from the sample. 2016 artículo científico 1516-635X https://www.redalyc.org/articulo.oa?id=179746750013 en http://www.redalyc.org/revista.oa?id=1797 Revista Brasileira de Ciência Avícola application/pdf Fundação APINCO de Ciência e Tecnologia Avícolas Revista Brasileira de Ciência Avícola (Brasil) Num.2 Vol.18 |
| title | Investigation of Influenza A, West Nile and Newcastle Disease Viruses in Birds from the Pantanal Wetlands of Mato Grosso, Brazil |
| topic | Agrociencias RT PCR qRT Virus Pantanal birds |
| url | https://www.redalyc.org/articulo.oa?id=179746750013 |