Catalytic properties of purified alpha amylase from Aspergillus flavus cultivated on low-cost agricultural substrate
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| Format: | Artículo científico |
| Language: | en |
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Universidad Nacional de Colombia
2023
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| _version_ | 1876452652214124544 |
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| author | Olushola E Owolabi |
| author_facet | Olushola E Owolabi |
| contents | Catalytic properties of purified alpha amylase from Aspergillus flavus cultivated on low-cost agricultural substrate Olushola E Owolabi Oladipo O Olaniyi Felix A Akinyosoye Agrociencias stability purification Alpha amylase characterization fermented cereals Aspergillus flavus isolated from fermented millet flour produced a crude enzyme, which was purified via ammonium sulphate precipitation and subsequent chromatographic techniques. The biochemical characteristics of the purified amylase were thereafter investigated showing activity in a wide range of pH and temperature, with optimal conditions of pH 6.0 and 50 °C. The enzyme retained even 89% of its activity after 1 h at 50 °C and 2 h at pH 6.0. The purified enzyme was stimulated by Ca2+, Zn2+ and Co2+, while Hg2+ and EDTA caused mild inhibition of α-amylase activity. The kinetic indices (Km and Vmax) and molecular weight of the enzyme were estimated in 1.71 mg mL-1, 2.133 μmol min-1 mL-1 and 45 kDa respectively. The catalytic properties of α-amylase from A. flavus makes it a promising candidate for use in various starch processing industries. 2023 artículo científico 0304-2847 https://www.redalyc.org/articulo.oa?id=179975180010 https://www.redalyc.org/journal/1799/179975180010/ https://www.redalyc.org/journal/1799/179975180010/html/ https://www.redalyc.org/journal/1799/179975180010/179975180010.epub https://www.redalyc.org/journal/1799/179975180010/movil 10.15446/rfnam.v76n1.100842 en http://www.redalyc.org/revista.oa?id=1799 Revista Facultad Nacional de Agronomía - Medellín application/pdf Universidad Nacional de Colombia Revista Facultad Nacional de Agronomía - Medellín (Colombia) Num.1 Vol.76 |
| format | Artículo científico |
| id | redalyc_179975180010 |
| institution | Redalyc |
| language | en |
| publishDate | 2023 |
| publisher | Universidad Nacional de Colombia |
| spellingShingle | Catalytic properties of purified alpha amylase from Aspergillus flavus cultivated on low-cost agricultural substrate Olushola E Owolabi Agrociencias stability purification Alpha amylase characterization fermented cereals Catalytic properties of purified alpha amylase from Aspergillus flavus cultivated on low-cost agricultural substrate Olushola E Owolabi Oladipo O Olaniyi Felix A Akinyosoye Agrociencias stability purification Alpha amylase characterization fermented cereals Aspergillus flavus isolated from fermented millet flour produced a crude enzyme, which was purified via ammonium sulphate precipitation and subsequent chromatographic techniques. The biochemical characteristics of the purified amylase were thereafter investigated showing activity in a wide range of pH and temperature, with optimal conditions of pH 6.0 and 50 °C. The enzyme retained even 89% of its activity after 1 h at 50 °C and 2 h at pH 6.0. The purified enzyme was stimulated by Ca2+, Zn2+ and Co2+, while Hg2+ and EDTA caused mild inhibition of α-amylase activity. The kinetic indices (Km and Vmax) and molecular weight of the enzyme were estimated in 1.71 mg mL-1, 2.133 μmol min-1 mL-1 and 45 kDa respectively. The catalytic properties of α-amylase from A. flavus makes it a promising candidate for use in various starch processing industries. 2023 artículo científico 0304-2847 https://www.redalyc.org/articulo.oa?id=179975180010 https://www.redalyc.org/journal/1799/179975180010/ https://www.redalyc.org/journal/1799/179975180010/html/ https://www.redalyc.org/journal/1799/179975180010/179975180010.epub https://www.redalyc.org/journal/1799/179975180010/movil 10.15446/rfnam.v76n1.100842 en http://www.redalyc.org/revista.oa?id=1799 Revista Facultad Nacional de Agronomía - Medellín application/pdf Universidad Nacional de Colombia Revista Facultad Nacional de Agronomía - Medellín (Colombia) Num.1 Vol.76 |
| title | Catalytic properties of purified alpha amylase from Aspergillus flavus cultivated on low-cost agricultural substrate |
| topic | Agrociencias stability purification Alpha amylase characterization fermented cereals |
| url | https://www.redalyc.org/articulo.oa?id=179975180010 https://www.redalyc.org/journal/1799/179975180010/ https://www.redalyc.org/journal/1799/179975180010/html/ https://www.redalyc.org/journal/1799/179975180010/179975180010.epub https://www.redalyc.org/journal/1799/179975180010/movil |