Effect of the combination of NAA, kinetin and sucrose on the induction of somatic embryogenesis in lulo ( Solanum quitoense Lam.)
Fuente:
Redalyc
Salvato in:
| Autore principale: | |
|---|---|
| Natura: | Artículo científico |
| Lingua: | en |
| Pubblicazione: |
Universidad Nacional de Colombia
2014
|
| Soggetti: | |
| Accesso online: | |
| Tags: |
Aggiungi Tag
Nessun Tag, puoi essere il primo ad aggiungerne!!
|
| _version_ | 1876433393364762624 |
|---|---|
| author | Hernando Criollo |
| author_facet | Hernando Criollo |
| contents | Effect of the combination of NAA, kinetin and sucrose on the induction of somatic embryogenesis in lulo ( Solanum quitoense Lam.) Hernando Criollo Margarita Perea Mariano Toribio Johanna Muñoz Agrociencias fruit recalcitrance Embryo culture tissue culture plant propagation Lulo is a species of great importance to the fruticulture of Colombia, but has significant phytosanitary problems that require an aggressive breeding program oriented toward the production of genotypes with tolerance to phytopathogens. These programs need to establish highly efficient mass plant propagation protocols, such as somatic embryogenesis. This study focused on research on the somatic embryogenesis of lulo using kinetin, naphthalene acetic acid-NAA (Plant Growth Regulators, PGRs), and different sucrose concentrations in a MS medium. Two lulo varieties, Solanum quitoense var. sep - tentrionale and S. quitoense var. quitoens e, and two explant types (hypocotyl and cotyledon) were used, incubated in dark conditions at 25±2°C. The highest production percentage of the embryos was obtained when 50 μM of NAA were added to the medium with sucrose (50.0 and 263.1 μM) for the two explant types used. In lulo with spines, the highest percentage of embryonic structures (50%) was observed with cotyledonary leaf explants and 50 μM of NAA; while in the spineless lulo, the embryonic structures were observed in the same type of explant with 50 μM of NAA + 263.1 μM of sucrose (32%). 2014 artículo científico 0120-9965 https://www.redalyc.org/articulo.oa?id=180332010003 en http://www.redalyc.org/revista.oa?id=1803 Agronomía Colombiana application/pdf Universidad Nacional de Colombia Agronomía Colombiana (Colombia) Num.2 Vol.32 |
| format | Artículo científico |
| id | redalyc_180332010003 |
| institution | Redalyc |
| language | en |
| publishDate | 2014 |
| publisher | Universidad Nacional de Colombia |
| spellingShingle | Effect of the combination of NAA, kinetin and sucrose on the induction of somatic embryogenesis in lulo ( Solanum quitoense Lam.) Hernando Criollo Agrociencias fruit recalcitrance Embryo culture tissue culture plant propagation Effect of the combination of NAA, kinetin and sucrose on the induction of somatic embryogenesis in lulo ( Solanum quitoense Lam.) Hernando Criollo Margarita Perea Mariano Toribio Johanna Muñoz Agrociencias fruit recalcitrance Embryo culture tissue culture plant propagation Lulo is a species of great importance to the fruticulture of Colombia, but has significant phytosanitary problems that require an aggressive breeding program oriented toward the production of genotypes with tolerance to phytopathogens. These programs need to establish highly efficient mass plant propagation protocols, such as somatic embryogenesis. This study focused on research on the somatic embryogenesis of lulo using kinetin, naphthalene acetic acid-NAA (Plant Growth Regulators, PGRs), and different sucrose concentrations in a MS medium. Two lulo varieties, Solanum quitoense var. sep - tentrionale and S. quitoense var. quitoens e, and two explant types (hypocotyl and cotyledon) were used, incubated in dark conditions at 25±2°C. The highest production percentage of the embryos was obtained when 50 μM of NAA were added to the medium with sucrose (50.0 and 263.1 μM) for the two explant types used. In lulo with spines, the highest percentage of embryonic structures (50%) was observed with cotyledonary leaf explants and 50 μM of NAA; while in the spineless lulo, the embryonic structures were observed in the same type of explant with 50 μM of NAA + 263.1 μM of sucrose (32%). 2014 artículo científico 0120-9965 https://www.redalyc.org/articulo.oa?id=180332010003 en http://www.redalyc.org/revista.oa?id=1803 Agronomía Colombiana application/pdf Universidad Nacional de Colombia Agronomía Colombiana (Colombia) Num.2 Vol.32 |
| title | Effect of the combination of NAA, kinetin and sucrose on the induction of somatic embryogenesis in lulo ( Solanum quitoense Lam.) |
| topic | Agrociencias fruit recalcitrance Embryo culture tissue culture plant propagation |
| url | https://www.redalyc.org/articulo.oa?id=180332010003 |