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| Formato: | Artículo científico |
| Lenguaje: | en |
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Universidad Nacional de Colombia
2020
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| Acceso en línea: | https://www.redalyc.org/articulo.oa?id=180376041007 https://www.redalyc.org/journal/1803/180376041007/ https://www.redalyc.org/journal/1803/180376041007/html/ https://www.redalyc.org/journal/1803/180376041007/180376041007.epub https://www.redalyc.org/journal/1803/180376041007/movil |
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| _version_ | 1866588813730512896 |
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| author | Diana Marcela Rivera-Toro |
| author_facet | Diana Marcela Rivera-Toro |
| contents | Detection and molecular characterization of the cucumber mosaic virus in chili pepper (Capsicum spp. L.) crops Diana Marcela Rivera-Toro Juan Carlos Vaca-Vaca Karina López-López Agrociencias PCR tabasco habanero Solanaceae Cucumovirus The chili pepper (Capsicum spp. L.) is a vegetable of economic importance that has been affected worldwide by the cucumber mosaic virus (CMV), a pathogen that causes a devastating disease in this crop. The aim of this research was the detection and characterization of CMV in chili pepper crops in Valle del Cauca, Colombia. Leaves of three chili pepper varieties (tabasco, cayenne and habanero) with viral symptoms were collected in four municipalities of Valle del Cauca. Total RNA was purified and a fragment of capsid protein (CP) from CMV was amplified by RT-PCR. Then, it was sequenced and bioinformatically analyzed, and from these sequences, specific primers were designed. From 71 chili pepper samples collected in Palmira, Yumbo, Vijes and Yotoco, 37 were positive for CMV (52.1%). The CMV chili pepper sequence analysis showed that they had their highest identity (98.5%) with a CMV isolated from bananas in Ecuador. Specific primers designed for CMV chili pepper showed greater sensitivity for detecting this virus (64.7% vs. 52.1%). The CMV chili pepper CP analysis indicated that it could be transmitted by the species Aphisgossypii. This study reports, for the first time, the molecular characterization of CMV in three chili pepper varieties. 2020 artículo científico 0120-9965 https://www.redalyc.org/articulo.oa?id=180376041007 https://www.redalyc.org/journal/1803/180376041007/ https://www.redalyc.org/journal/1803/180376041007/html/ https://www.redalyc.org/journal/1803/180376041007/180376041007.epub https://www.redalyc.org/journal/1803/180376041007/movil 10.15446/agron.colomb.v38n2.82975 en http://www.redalyc.org/revista.oa?id=1803 Agronomía Colombiana application/pdf Universidad Nacional de Colombia Agronomía Colombiana (Colombia) Num.2 Vol.38 |
| format | Artículo científico |
| id | redalyc_180376041007 |
| language | en |
| publishDate | 2020 |
| publisher | Universidad Nacional de Colombia |
| spellingShingle | Detection and molecular characterization of the cucumber mosaic virus in chili pepper (Capsicum spp. L.) crops Diana Marcela Rivera-Toro Agrociencias PCR tabasco habanero Solanaceae Cucumovirus Detection and molecular characterization of the cucumber mosaic virus in chili pepper (Capsicum spp. L.) crops Diana Marcela Rivera-Toro Juan Carlos Vaca-Vaca Karina López-López Agrociencias PCR tabasco habanero Solanaceae Cucumovirus The chili pepper (Capsicum spp. L.) is a vegetable of economic importance that has been affected worldwide by the cucumber mosaic virus (CMV), a pathogen that causes a devastating disease in this crop. The aim of this research was the detection and characterization of CMV in chili pepper crops in Valle del Cauca, Colombia. Leaves of three chili pepper varieties (tabasco, cayenne and habanero) with viral symptoms were collected in four municipalities of Valle del Cauca. Total RNA was purified and a fragment of capsid protein (CP) from CMV was amplified by RT-PCR. Then, it was sequenced and bioinformatically analyzed, and from these sequences, specific primers were designed. From 71 chili pepper samples collected in Palmira, Yumbo, Vijes and Yotoco, 37 were positive for CMV (52.1%). The CMV chili pepper sequence analysis showed that they had their highest identity (98.5%) with a CMV isolated from bananas in Ecuador. Specific primers designed for CMV chili pepper showed greater sensitivity for detecting this virus (64.7% vs. 52.1%). The CMV chili pepper CP analysis indicated that it could be transmitted by the species Aphisgossypii. This study reports, for the first time, the molecular characterization of CMV in three chili pepper varieties. 2020 artículo científico 0120-9965 https://www.redalyc.org/articulo.oa?id=180376041007 https://www.redalyc.org/journal/1803/180376041007/ https://www.redalyc.org/journal/1803/180376041007/html/ https://www.redalyc.org/journal/1803/180376041007/180376041007.epub https://www.redalyc.org/journal/1803/180376041007/movil 10.15446/agron.colomb.v38n2.82975 en http://www.redalyc.org/revista.oa?id=1803 Agronomía Colombiana application/pdf Universidad Nacional de Colombia Agronomía Colombiana (Colombia) Num.2 Vol.38 |
| title | Detection and molecular characterization of the cucumber mosaic virus in chili pepper (Capsicum spp. L.) crops |
| topic | Agrociencias PCR tabasco habanero Solanaceae Cucumovirus |
| url | https://www.redalyc.org/articulo.oa?id=180376041007 https://www.redalyc.org/journal/1803/180376041007/ https://www.redalyc.org/journal/1803/180376041007/html/ https://www.redalyc.org/journal/1803/180376041007/180376041007.epub https://www.redalyc.org/journal/1803/180376041007/movil |