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| Format: | Artículo científico |
| Langue: | en |
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Centro Nacional de Investigaciones Científicas
2006
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| Accès en ligne: | https://www.redalyc.org/articulo.oa?id=181220529013 |
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- Poly(L-lactic acid) scaffold: in vitro degradation in alkaline medium, phosphate buffer solution and osteoblast morphology Samuel Hilsdorf Barbanti Cecília Amélia de Carvalho Zavaglia Arnaldo Santos Jr. Eliana Aparecida de Rezende Duek Biología poly(L biomaterial lactic acid) cell culture biodegradation Poly(L-lactic acid) (PLLA) is a biodegradable and bioresorbable polymer widely studied for biomedical applications as an alternative for the treatment of lesions and the loss of tissue. As a temporary biomaterial, the degradation of the PLLA occurs by hydrolysis, and the products of degradation (carbon dioxide and water) are metabolized by the organism. The aim of this work was to obtain, to characterize and to evaluate the in vitro degradation of dense and porous PLLA membranes. Dense membranes were prepared dissolving the polymer in chloroform in 10 % (w/v), at room temperature. The solution was transferred to glass plates and casting made the drying procedure. Porous membranes were prepared in similar way described previously. After the dissolution, citrate sodic was included, concentration of 50 % (w/v), with the salt diameter previously sieved (grain diameter < 45 mm, 180-250 mm and 250-350 mm). After the casting, the salt was removed by the immersion in water distilled. The samples were vacuum dried and maintained in a dessicator to guarantee the total removal of solvent. Using different molar mass (Mw = 300 000 and 100 000) and degradation medium (phosphate buffer solution (PBS) and alkaline) the degradation process was evaluated by differential scanning calorimetry (DSC) and scanning electron microscopy (SEM). The cultured osteoblastic cells was evaluated by the morphology using SEM. Compared with the PBS, alkaline medium offers significant acceleration of the degradation process. The morphology of the degradation process confirms the autocatalytic effects of poly(alpha-hydroxy acids), in which the degradation is more accentuated in the dense structures due to the concentration of the acid products inside the material. Data of osteoblasts culture showed similar morphology compared with the control. The selection of the materials for the Tissue Engineering is dependent on the application and the structures of PLLA could be suitable in the cases where the lesion demanded a material to degrade in a long time serving as physical support for the cells and mechanical support for the tissue. 2006 artículo científico 0253-5688 https://www.redalyc.org/articulo.oa?id=181220529013 en http://www.redalyc.org/revista.oa?id=1812 Revista CENIC. Ciencias Biológicas application/pdf Centro Nacional de Investigaciones Científicas Revista CENIC. Ciencias Biológicas (Cuba) Num.3 Vol.37