The genome of Vibrio cholerae contains two different and functional genes for aspartate semialdehyde dehydrogenases
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Centro Nacional de Investigaciones Científicas
2004
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| _version_ | 1876480494264123392 |
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| author | Karen Marrero |
| author_facet | Karen Marrero |
| contents | The genome of Vibrio cholerae contains two different and functional genes for aspartate semialdehyde dehydrogenases Karen Marrero Emilio Marrero Yussuan Silva Boris Rodríguez Javier Campos Talena Ledón Edgar Valle Rafael Fando Biología VC2036 VC2107 asd mutants Vibrio cholerae aspartate semialdehyde dehydrogenase Two asd genes were identified in Vibrio cholerae and cloned in E. coli on the basis of their ability to complement the Asd defect of strain c6097. The aspartate semialdehyde dehydrogenase activity of each gene product was also confirmed in vitro. Although both genes were able to complement the asd defect in E. coli, they were of different size and no important homology was found between them. The nucleotide sequences and the predicted polypeptides were established and compared with the sequences deposited in the Genbank and the Swissprot, respectively. Each gene product belongs to one of two groups of homology found for Asd in the Swissprot. The asd genes found in this work corresponded to genes VC2036 and VC2107 of the chromosome I of the Vibrio cholerae genome deposited at the TIGR. Mutants in each of these genes were obtained and characterized microbiologically, but the double mutant was only attained in the presence of a trans-complementing asd gene. This restriction to generate the double mutant was found to reflect the absence of an efficient mechanism for diaminopimelic acid (DAP) transport in Vibrio cholerae. The in vivo and in vitro behavior of the constructed mutants was also evaluated in animal models and under conditions of laboratory. 2004 artículo científico 0253-5688 https://www.redalyc.org/articulo.oa?id=181225915005 en http://www.redalyc.org/revista.oa?id=1812 Revista CENIC. Ciencias Biológicas application/pdf Centro Nacional de Investigaciones Científicas Revista CENIC. Ciencias Biológicas (Cuba) Num.3 Vol.35 |
| format | Artículo científico |
| id | redalyc_181225915005 |
| institution | Redalyc |
| language | en |
| publishDate | 2004 |
| publisher | Centro Nacional de Investigaciones Científicas |
| spellingShingle | The genome of Vibrio cholerae contains two different and functional genes for aspartate semialdehyde dehydrogenases Karen Marrero Biología VC2036 VC2107 asd mutants Vibrio cholerae aspartate semialdehyde dehydrogenase The genome of Vibrio cholerae contains two different and functional genes for aspartate semialdehyde dehydrogenases Karen Marrero Emilio Marrero Yussuan Silva Boris Rodríguez Javier Campos Talena Ledón Edgar Valle Rafael Fando Biología VC2036 VC2107 asd mutants Vibrio cholerae aspartate semialdehyde dehydrogenase Two asd genes were identified in Vibrio cholerae and cloned in E. coli on the basis of their ability to complement the Asd defect of strain c6097. The aspartate semialdehyde dehydrogenase activity of each gene product was also confirmed in vitro. Although both genes were able to complement the asd defect in E. coli, they were of different size and no important homology was found between them. The nucleotide sequences and the predicted polypeptides were established and compared with the sequences deposited in the Genbank and the Swissprot, respectively. Each gene product belongs to one of two groups of homology found for Asd in the Swissprot. The asd genes found in this work corresponded to genes VC2036 and VC2107 of the chromosome I of the Vibrio cholerae genome deposited at the TIGR. Mutants in each of these genes were obtained and characterized microbiologically, but the double mutant was only attained in the presence of a trans-complementing asd gene. This restriction to generate the double mutant was found to reflect the absence of an efficient mechanism for diaminopimelic acid (DAP) transport in Vibrio cholerae. The in vivo and in vitro behavior of the constructed mutants was also evaluated in animal models and under conditions of laboratory. 2004 artículo científico 0253-5688 https://www.redalyc.org/articulo.oa?id=181225915005 en http://www.redalyc.org/revista.oa?id=1812 Revista CENIC. Ciencias Biológicas application/pdf Centro Nacional de Investigaciones Científicas Revista CENIC. Ciencias Biológicas (Cuba) Num.3 Vol.35 |
| title | The genome of Vibrio cholerae contains two different and functional genes for aspartate semialdehyde dehydrogenases |
| topic | Biología VC2036 VC2107 asd mutants Vibrio cholerae aspartate semialdehyde dehydrogenase |
| url | https://www.redalyc.org/articulo.oa?id=181225915005 |