Saved in:
| Main Author: | |
|---|---|
| Format: | Artículo científico |
| Language: | en |
| Published: |
Universidade Estadual de Maringá
2011
|
| Subjects: | |
| Online Access: | https://www.redalyc.org/articulo.oa?id=187121352004 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
Table of Contents:
- Effect of alcohol and formaldehyde on the S13C and S15N isotopic composition of Plagioscion squamosissimus and Hypophthalmus edentates (Pisces, Osteichthyes) Gislaine Iachstel Manetta Evanilde Benedito Carlos Ducatti Biología fishes preservative Stable isotope Hypophthalmus edentatus Plagioscion squamosissimus The present study investigates the S13C and S15N isotopic composition in frozen samples (control), samples in alcohol and in formaldehyde of Plagioscion squamosissimus and Hypophthalmus edentatus. From each individual we extracted a strip of muscle from the region above the lateral line, in the dorsal fin base, that was divided into three equal parts, each one was submitted to one type of treatment: freeze - control group (-15oC), conservation in alcohol 70% and fixation in formaldehyde 4%. Samples were kept under those treatments for 30 days, washed and submerged in distilled water for 4 hours. Afterwards, they were dried up in air oven at 60oC for 48 hours and macerated until the obtaining of a fine powder. A significant difference was found in isotopic values of carbon and nitrogen, between the control and the samples in alcohol and formaldehyde, except for S13C from the H. edentatus samples in formaldehyde. The carbon isotopic values of samples in alcohol were mostly enriched compared to control, whereas the samples in formaldehyde presented depleted values in relation to the control. The nitrogen isotopic values for both samples preserved in alcohol and formaldehyde were enriched when compared to the values of frozen samples, independently of used preservatives. Therefore, the isotopic correction should be accomplished according to the isotope and preservative employed for species of freshwater fish. 2011 artículo científico 1679-9283 https://www.redalyc.org/articulo.oa?id=187121352004 en http://www.redalyc.org/revista.oa?id=1871 Acta Scientiarum. Biological Sciences application/pdf Universidade Estadual de Maringá Acta Scientiarum. Biological Sciences (Brasil) Num.4 Vol.33