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| Format: | Artículo científico |
| Language: | en |
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Universidade Estadual de Maringá
2014
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| Online Access: | https://www.redalyc.org/articulo.oa?id=187129844004 |
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Table of Contents:
- Establishment and content of sugars and phenols in Physalis callus obtained from different explants and concentrations of bap and naa Douglas Junior Bertoncelli Marisa de Cacia Oliveira Amanda Izabel dos Passos Ana Claudia Ariati Alexandre Osmar Ortolan Elaine de Paula Witt Biología cell growth sugars phenols Keywords Obtaining cells of Physalis pubescens is of interest for studies of primary and secondary metabolic pathways, in the search for new active molecules. Our objectives were to evaluate the regeneration potential of explants from different parts of the plant, growth regulators to be used, and the determination of the growth curve of the callus. We used explants of leaf, root, stem and petiole, cultured on Murashige and Skoog medium with differen t concentrations of 6- benzylaminopurine and - naphthaleneacetic acid. The explants from stem and peti ole had a higher regeneration potential of the shoot to the treatment with 0.5 mg L -1 6-benzylaminopurine, and the explan ts of leave and r oot emitted more roots, with lower production of call us. The tests showed that the regenera tion of the whole plant should be done in two steps: cultivation for shoot regeneration and transplantation to a new rooting medium. The growth of callus showed five distinct phases, with accumulation of phen ols in the final stages of growth. The levels of soluble sugars increased with age, wh ile reducing sugars showed variations, with higher concentrations in the initial stages of cultivation, with fall and rise again at the final evaluation (28 th day). 2014 artículo científico 1679-9283 https://www.redalyc.org/articulo.oa?id=187129844004 en http://www.redalyc.org/revista.oa?id=1871 Acta Scientiarum. Biological Sciences application/pdf Universidade Estadual de Maringá Acta Scientiarum. Biological Sciences (Brasil) Num.1 Vol.36