In vitro propagation of Vriesea reitzii, a native epiphyte bromeliad from the Atlantic rainforest
Fuente:
Redalyc
Enregistré dans:
| Auteur principal: | |
|---|---|
| Format: | Artículo científico |
| Langue: | en |
| Publié: |
Universidade Estadual de Maringá
2014
|
| Sujets: | |
| Accès en ligne: | |
| Tags: |
Ajouter un tag
Pas de tags, Soyez le premier à ajouter un tag!
|
| _version_ | 1876480813122453504 |
|---|---|
| author | Lírio Luiz Dal Vesco |
| author_facet | Lírio Luiz Dal Vesco |
| contents | In vitro propagation of Vriesea reitzii, a native epiphyte bromeliad from the Atlantic rainforest Lírio Luiz Dal Vesco Rosete Pescador Jenny Paola Corredor Prado Leocir José Welter Miguel Pedro Guerra Biología conservation regeneration nodular culture Adventitious shoots The induction of nodular culture (NC) and the subsequent development of microshoots of V. reitzii are considered an in vitro propagation model-system with high regenerative performance. Current research analyzed the determinant factors of the in vitro morphogenesis control of bromeliads. Seeds excised from mature capsules were grown on medium MS basic (MSB), liquid or gelled, supplemented or not with α-naphthaleneacetic acid (NAA), 6-benzilaminopurine (BAP) or thidiazuron (TDZ). The regeneration and elongation of microshoots were evaluated from NC sub-cultivated on MSB medium on liquid culture medium supplemented with different concentrations of indolyl-3-acetic acid (IAA) and gibberellic acid (GA3). Plant growth regulators (PGR) supplemented into the medium MSB inhibited the germination of the seeds and induced NC in the second week of growth. The induced NC on MSB medium with NAA (4 μM) and sub-cultivated on MSB medium with NAA (2 μM) plus N6(2-isopentenyl) adenine (2-iP) (2 μM) showed granular texture and high rate of proliferation. NC sub-culture in MSB medium with IAA (4 μM) provided a higher average number of microshoots (1,478 shoots g-1 of NC). Shoots over 3.0 cm resulted in more than 95% ex vitro survival. 2014 artículo científico 1679-9283 https://www.redalyc.org/articulo.oa?id=187131652002 en http://www.redalyc.org/revista.oa?id=1871 Acta Scientiarum. Biological Sciences application/pdf Universidade Estadual de Maringá Acta Scientiarum. Biological Sciences (Brasil) Num.3 Vol.36 |
| format | Artículo científico |
| id | redalyc_187131652002 |
| institution | Redalyc |
| language | en |
| publishDate | 2014 |
| publisher | Universidade Estadual de Maringá |
| spellingShingle | In vitro propagation of Vriesea reitzii, a native epiphyte bromeliad from the Atlantic rainforest Lírio Luiz Dal Vesco Biología conservation regeneration nodular culture Adventitious shoots In vitro propagation of Vriesea reitzii, a native epiphyte bromeliad from the Atlantic rainforest Lírio Luiz Dal Vesco Rosete Pescador Jenny Paola Corredor Prado Leocir José Welter Miguel Pedro Guerra Biología conservation regeneration nodular culture Adventitious shoots The induction of nodular culture (NC) and the subsequent development of microshoots of V. reitzii are considered an in vitro propagation model-system with high regenerative performance. Current research analyzed the determinant factors of the in vitro morphogenesis control of bromeliads. Seeds excised from mature capsules were grown on medium MS basic (MSB), liquid or gelled, supplemented or not with α-naphthaleneacetic acid (NAA), 6-benzilaminopurine (BAP) or thidiazuron (TDZ). The regeneration and elongation of microshoots were evaluated from NC sub-cultivated on MSB medium on liquid culture medium supplemented with different concentrations of indolyl-3-acetic acid (IAA) and gibberellic acid (GA3). Plant growth regulators (PGR) supplemented into the medium MSB inhibited the germination of the seeds and induced NC in the second week of growth. The induced NC on MSB medium with NAA (4 μM) and sub-cultivated on MSB medium with NAA (2 μM) plus N6(2-isopentenyl) adenine (2-iP) (2 μM) showed granular texture and high rate of proliferation. NC sub-culture in MSB medium with IAA (4 μM) provided a higher average number of microshoots (1,478 shoots g-1 of NC). Shoots over 3.0 cm resulted in more than 95% ex vitro survival. 2014 artículo científico 1679-9283 https://www.redalyc.org/articulo.oa?id=187131652002 en http://www.redalyc.org/revista.oa?id=1871 Acta Scientiarum. Biological Sciences application/pdf Universidade Estadual de Maringá Acta Scientiarum. Biological Sciences (Brasil) Num.3 Vol.36 |
| title | In vitro propagation of Vriesea reitzii, a native epiphyte bromeliad from the Atlantic rainforest |
| topic | Biología conservation regeneration nodular culture Adventitious shoots |
| url | https://www.redalyc.org/articulo.oa?id=187131652002 |