Inhibition of coagulation proteases and thrombosis and sub-chronic toxicological study of a sulfated polysaccharidic fraction from the red alga Gelidiella acerosa
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| Format: | Artículo científico |
| Langue: | en |
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Universidade Estadual de Maringá
2014
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| _version_ | 1876472845643546624 |
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| author | Ismael Nilo Lino de Queiroz |
| author_facet | Ismael Nilo Lino de Queiroz |
| contents | Inhibition of coagulation proteases and thrombosis and sub-chronic toxicological study of a sulfated polysaccharidic fraction from the red alga Gelidiella acerosa Ismael Nilo Lino de Queiroz José Ariévilo Gurgel Rodrigues Ana Luíza Gomes Quinderé Márjory Lima Holanda Maria Gonçalves Pereira Norma Maria Barros Benevides Biología Keywords Rhodophyta polysulfated bioactivities in vivo toxicity Metabolites isolated from Gelidiella species (Rhodophyta) have been few studied. We evaluated a sulfated polysaccharidic fraction from G. acerosa collected from two Br azilian beaches on the northwestern coast of Brazil (Flecheiras-F and Pedra Rachada-PR) on coagulation proteases and thrombosis. Their toxicity in vivo was also assessed. Enzymatic extr actions yielded 1.40%, and similar chromatographic profiles (DEAE-cellulose ) were obtained, with fractions (Ga-I → V) containing differences among the relative proportions of su lfate (5-42%), and revealing charge density patterns by electrophoresis. Ga-IV-PR had a discrete effect (3.01 IU mg -1 ) on normal human coagulation compared with heparin (193 IU mg -1 ) and was tested on coagulatio n proteases (thrombin and factor Xa) in the presence of antithrombin and in a model of venous thrombosis in rats using thromboplastin as the thrombogenic stimulus. The systems were inhibited; but at higher doses (>1.0 mg kg -1 ), this fraction reverted the antithrombotic effect. Regarding the toxicological study, consecutive Ga-IV (9 mg kg -1 ) for 14 days did not cause mortality in mice, but some biochemical and hematological parameters were discretely altered. Histopathological analysis revealed that increased liver and spleen sizes had no toxicological significance. Therefore, G. acerosa does not biochemically change its matrix polysaccharide composition and proved to be safe antithrombotic agent. 2014 artículo científico 1679-9283 https://www.redalyc.org/articulo.oa?id=187132092003 en http://www.redalyc.org/revista.oa?id=1871 Acta Scientiarum. Biological Sciences application/pdf Universidade Estadual de Maringá Acta Scientiarum. Biological Sciences (Brasil) Num.4 Vol.36 |
| format | Artículo científico |
| id | redalyc_187132092003 |
| institution | Redalyc |
| language | en |
| publishDate | 2014 |
| publisher | Universidade Estadual de Maringá |
| spellingShingle | Inhibition of coagulation proteases and thrombosis and sub-chronic toxicological study of a sulfated polysaccharidic fraction from the red alga Gelidiella acerosa Ismael Nilo Lino de Queiroz Biología Keywords Rhodophyta polysulfated bioactivities in vivo toxicity Inhibition of coagulation proteases and thrombosis and sub-chronic toxicological study of a sulfated polysaccharidic fraction from the red alga Gelidiella acerosa Ismael Nilo Lino de Queiroz José Ariévilo Gurgel Rodrigues Ana Luíza Gomes Quinderé Márjory Lima Holanda Maria Gonçalves Pereira Norma Maria Barros Benevides Biología Keywords Rhodophyta polysulfated bioactivities in vivo toxicity Metabolites isolated from Gelidiella species (Rhodophyta) have been few studied. We evaluated a sulfated polysaccharidic fraction from G. acerosa collected from two Br azilian beaches on the northwestern coast of Brazil (Flecheiras-F and Pedra Rachada-PR) on coagulation proteases and thrombosis. Their toxicity in vivo was also assessed. Enzymatic extr actions yielded 1.40%, and similar chromatographic profiles (DEAE-cellulose ) were obtained, with fractions (Ga-I → V) containing differences among the relative proportions of su lfate (5-42%), and revealing charge density patterns by electrophoresis. Ga-IV-PR had a discrete effect (3.01 IU mg -1 ) on normal human coagulation compared with heparin (193 IU mg -1 ) and was tested on coagulatio n proteases (thrombin and factor Xa) in the presence of antithrombin and in a model of venous thrombosis in rats using thromboplastin as the thrombogenic stimulus. The systems were inhibited; but at higher doses (>1.0 mg kg -1 ), this fraction reverted the antithrombotic effect. Regarding the toxicological study, consecutive Ga-IV (9 mg kg -1 ) for 14 days did not cause mortality in mice, but some biochemical and hematological parameters were discretely altered. Histopathological analysis revealed that increased liver and spleen sizes had no toxicological significance. Therefore, G. acerosa does not biochemically change its matrix polysaccharide composition and proved to be safe antithrombotic agent. 2014 artículo científico 1679-9283 https://www.redalyc.org/articulo.oa?id=187132092003 en http://www.redalyc.org/revista.oa?id=1871 Acta Scientiarum. Biological Sciences application/pdf Universidade Estadual de Maringá Acta Scientiarum. Biological Sciences (Brasil) Num.4 Vol.36 |
| title | Inhibition of coagulation proteases and thrombosis and sub-chronic toxicological study of a sulfated polysaccharidic fraction from the red alga Gelidiella acerosa |
| topic | Biología Keywords Rhodophyta polysulfated bioactivities in vivo toxicity |
| url | https://www.redalyc.org/articulo.oa?id=187132092003 |