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| Format: | Artículo científico |
| Sprache: | en |
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Universidade Estadual de Maringá
2015
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| Online-Zugang: | https://www.redalyc.org/articulo.oa?id=187143301009 |
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- Esterase isozymes patterns of grape vine (Vitis vinifera L.) are altered in response to fungicide exposure Gleice Ribeiro Orasmo Sandra Aparecida de Oliveira-Collet Claudete Aparecida Mangolin Ana Sílvia Lapenta Maria de Fátima Pires da Silva Machado Biología isozymes Esterases fungicides vine clones differential patterns Current analysis characterizes the effect of different fungicides often applied for pest control on α−and β-esterase patterns of four economically important table-wine grape cultivars (Italia, Rubi, Benitaka and Brasil) of Vitis vinifera. The α- and β-esterase patterns in bud leaves of the cultivars were assessed by native PAGE analysis. Cabrio Top® compound inhibited EST-2, EST-5, EST-6, EST-7, EST-8, EST-9 and EST-10 carboxylesterases, whereas EST-4, EST-11, EST-12, EST-13, EST-14 acetylesterases and EST-16 carboxylesterase were detected as weakly stained bands. Carboxylesterases and acetylesterases were also detected as weakly stained bands when exposed to fungicides Orthocide 500®, Positron Duo® and Folicur PM®. No changes in α- and β- esterase patterns were reported when the vines were exposed to the fungicides Rovral SC®, Kumulus DF®, Curzate M®, Score® or Cuprogarb 500®. The evidence of functional changes in carboxylesterase and acetylesterase levels in current study is a warning to grape producers on the dangers inherent in the indiscriminate use of potent and modern fungicides extensively used in agriculture. The inhibition effect of fungicides on esterase isozyme molecules seems to be independent of the fungicide chemical. 2015 artículo científico 1679-9283 https://www.redalyc.org/articulo.oa?id=187143301009 en http://www.redalyc.org/revista.oa?id=1871 Acta Scientiarum. Biological Sciences application/pdf Universidade Estadual de Maringá Acta Scientiarum. Biological Sciences (Brasil) Num.4 Vol.37