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| Main Author: | |
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| Format: | Artículo científico |
| Language: | en |
| Published: |
Instituto Nacional de Ciencias Agrícolas
2005
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| Subjects: | |
| Online Access: | https://www.redalyc.org/articulo.oa?id=193215916004 |
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Table of Contents:
- USE OF cDNA MICROARRAY TECHNOLOGY FOR IDENTIFICATION OF NOVEL GENES RESPONDING TO ABSCISIC ACID PHYTOHORMONE D. Cabezas Sandra Pérez R. Huelva Dong Haitao Li Debao Agrociencias ABA cytochrome C Molecular genetics DNA microarray technology is a new and powerful technology that will substantially increase the speed of molecular biological research. This paper gives a survey of DNA microarray technology and its use in gene expression. Studies of 4370 unigenes from a library of rice endosperm and leaf tissue were used to detect the expression levels of mRNA from rice stem tissue treated by water and plant hormone ABA. Results showed that the expression levels of five genes were depressed by phytohormone ABA. Reverse Northern blotting confirmed that one of the five genes H024g06 was really depressed by ABA. Bioinformatic analysis showed that the gene H024g06 is the same as the cytochrome C gene. Previous researches revealed that the cytochrome C gene was related to such stress responses as drought and coldness, while ABA could induce plant stress response. Therefore, these results suggest that the cytochrome C gene may have a certain mediating function during the stress response induced by ABA phytohormone. 2005 artículo científico 1819-4087 https://www.redalyc.org/articulo.oa?id=193215916004 en http://www.redalyc.org/revista.oa?id=1932 Cultivos Tropicales application/pdf Instituto Nacional de Ciencias Agrícolas Cultivos Tropicales (Cuba) Num.1 Vol.26