HIDRÓLISIS ENZIMÁTICA DE QUITOSANA. ACTIVIDAD BIOLÓGICA DEL POLÍMERO Y SUS HIDROLIZADOS
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| Format: | Artículo científico |
| Sprache: | es |
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Instituto Nacional de Ciencias Agrícolas
2004
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| _version_ | 1876430885582012416 |
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| author | A. Falcón |
| author_facet | A. Falcón |
| contents | HIDRÓLISIS ENZIMÁTICA DE QUITOSANA. ACTIVIDAD BIOLÓGICA DEL POLÍMERO Y SUS HIDROLIZADOS A. Falcón D. Díaz M. A. Ramírez Agrociencias Chitosan fungal diseases chitinase activity enzymatic hydrolysis Phytophthora parasitica Chitosan is a chitin-derived desacetylate, a polymer of N-acetil-Glucosamine that is extracted from the exoskeleton of the crustaceans. Both polymers as well as their oligomers have proved to protect several species of plants against fungal diseases by means of the induction of defensive mechanisms that stop the advance of the pathogen in the vegetable and by direct action of pathogenic fungi growth inhibition. In this work, the hydrolysis of chitosan with Pectinex Ultra SPL (Novozymes) was carried out and the course of the reaction was monitored by determining the intrinsic viscosity of the solution as well as the increment of reducing sugars during the reaction time. The analysis of hydrolysis results allowed to suggest a remarkable endo-chitosanase character for the Pectinex enzymatic pool. Additionally, the activity of in vitro growth inhibition of the pathogen Phytophthora parasitica and the inducing chitinase activity in soybean plants was comparatively studied, using the native chitosan and their enzymatic hydrolysates of two and 10 hours. A bigger growth inhibition of P. parasitica was observed in the case of hydrolysates regarding the native chitosan, as well as a higher induction of chitinase activity in soybean plants previously treated with the 10 hour-hydrolysate. Results are discussed concerning the importance of GP in the antifungal activity and signal perception that induces soybean plant defense response, taking into account the increment of the number of fragments by means of the hydrolysis. 2004 artículo científico 1819-4087 https://www.redalyc.org/articulo.oa?id=193217832012 es http://www.redalyc.org/revista.oa?id=1932 Cultivos Tropicales application/pdf Instituto Nacional de Ciencias Agrícolas Cultivos Tropicales (Cuba) Num.2 Vol.25 |
| format | Artículo científico |
| id | redalyc_193217832012 |
| institution | Redalyc |
| language | es |
| publishDate | 2004 |
| publisher | Instituto Nacional de Ciencias Agrícolas |
| spellingShingle | HIDRÓLISIS ENZIMÁTICA DE QUITOSANA. ACTIVIDAD BIOLÓGICA DEL POLÍMERO Y SUS HIDROLIZADOS A. Falcón Agrociencias Chitosan fungal diseases chitinase activity enzymatic hydrolysis Phytophthora parasitica HIDRÓLISIS ENZIMÁTICA DE QUITOSANA. ACTIVIDAD BIOLÓGICA DEL POLÍMERO Y SUS HIDROLIZADOS A. Falcón D. Díaz M. A. Ramírez Agrociencias Chitosan fungal diseases chitinase activity enzymatic hydrolysis Phytophthora parasitica Chitosan is a chitin-derived desacetylate, a polymer of N-acetil-Glucosamine that is extracted from the exoskeleton of the crustaceans. Both polymers as well as their oligomers have proved to protect several species of plants against fungal diseases by means of the induction of defensive mechanisms that stop the advance of the pathogen in the vegetable and by direct action of pathogenic fungi growth inhibition. In this work, the hydrolysis of chitosan with Pectinex Ultra SPL (Novozymes) was carried out and the course of the reaction was monitored by determining the intrinsic viscosity of the solution as well as the increment of reducing sugars during the reaction time. The analysis of hydrolysis results allowed to suggest a remarkable endo-chitosanase character for the Pectinex enzymatic pool. Additionally, the activity of in vitro growth inhibition of the pathogen Phytophthora parasitica and the inducing chitinase activity in soybean plants was comparatively studied, using the native chitosan and their enzymatic hydrolysates of two and 10 hours. A bigger growth inhibition of P. parasitica was observed in the case of hydrolysates regarding the native chitosan, as well as a higher induction of chitinase activity in soybean plants previously treated with the 10 hour-hydrolysate. Results are discussed concerning the importance of GP in the antifungal activity and signal perception that induces soybean plant defense response, taking into account the increment of the number of fragments by means of the hydrolysis. 2004 artículo científico 1819-4087 https://www.redalyc.org/articulo.oa?id=193217832012 es http://www.redalyc.org/revista.oa?id=1932 Cultivos Tropicales application/pdf Instituto Nacional de Ciencias Agrícolas Cultivos Tropicales (Cuba) Num.2 Vol.25 |
| title | HIDRÓLISIS ENZIMÁTICA DE QUITOSANA. ACTIVIDAD BIOLÓGICA DEL POLÍMERO Y SUS HIDROLIZADOS |
| topic | Agrociencias Chitosan fungal diseases chitinase activity enzymatic hydrolysis Phytophthora parasitica |
| url | https://www.redalyc.org/articulo.oa?id=193217832012 |