ALTERNATIVA DE LA TÉCNICA DE TINCIÓN PARA DETERMINAR LA COLONIZACIÓN MICORRÍZICA
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| Format: | Artículo científico |
| Langue: | es |
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Instituto Nacional de Ciencias Agrícolas
2015
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| _version_ | 1876436557529874432 |
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| author | Yakelín Rodríguez Yon |
| author_facet | Yakelín Rodríguez Yon |
| contents | ALTERNATIVA DE LA TÉCNICA DE TINCIÓN PARA DETERMINAR LA COLONIZACIÓN MICORRÍZICA Yakelín Rodríguez Yon Lianne Arias Pérez Aida Medina Carmona Yonaisy Mujica Pérez Laura R. Medina García Kalyanne Fernández Suárez Aracely Mena Echevarría Agrociencias roots colorants health protection Arbuscular mycorrhiza The root stainning technique is neccesary in the work with arbuscular mycorrhizal fungi (AMF), which constitute the base of several Biofertilizers, as the EcoMic® product, each time more used on agricultural practices at world level. That`s why the sample number evaluated at Mycorrhiza laboratories from diverse Research, Academic and even some productive Centers, increases every year. Those works require the application of such technique; nevertheless, it has the inconvenient to use highly toxic and carcinogenic reagents. The objective of the present work was to substitute those reagents by others non harmful to human health and environment. This is the case of Trypan blue that was substituted by the washable pen ink Parker QuinK. Moreover, Lactic acid and Glycerin were eliminated from the protocol. Results showed the pertinence of such modifications and revealed the usefulness and high quality of mentioned ink, which successfully stains all fungal structures allowing a clear visualization during long time. 2015 artículo científico 1819-4087 https://www.redalyc.org/articulo.oa?id=193239249003 es http://www.redalyc.org/revista.oa?id=1932 Cultivos Tropicales application/pdf Instituto Nacional de Ciencias Agrícolas Cultivos Tropicales (Cuba) Num.2 Vol.36 |
| format | Artículo científico |
| id | redalyc_193239249003 |
| institution | Redalyc |
| language | es |
| publishDate | 2015 |
| publisher | Instituto Nacional de Ciencias Agrícolas |
| spellingShingle | ALTERNATIVA DE LA TÉCNICA DE TINCIÓN PARA DETERMINAR LA COLONIZACIÓN MICORRÍZICA Yakelín Rodríguez Yon Agrociencias roots colorants health protection Arbuscular mycorrhiza ALTERNATIVA DE LA TÉCNICA DE TINCIÓN PARA DETERMINAR LA COLONIZACIÓN MICORRÍZICA Yakelín Rodríguez Yon Lianne Arias Pérez Aida Medina Carmona Yonaisy Mujica Pérez Laura R. Medina García Kalyanne Fernández Suárez Aracely Mena Echevarría Agrociencias roots colorants health protection Arbuscular mycorrhiza The root stainning technique is neccesary in the work with arbuscular mycorrhizal fungi (AMF), which constitute the base of several Biofertilizers, as the EcoMic® product, each time more used on agricultural practices at world level. That`s why the sample number evaluated at Mycorrhiza laboratories from diverse Research, Academic and even some productive Centers, increases every year. Those works require the application of such technique; nevertheless, it has the inconvenient to use highly toxic and carcinogenic reagents. The objective of the present work was to substitute those reagents by others non harmful to human health and environment. This is the case of Trypan blue that was substituted by the washable pen ink Parker QuinK. Moreover, Lactic acid and Glycerin were eliminated from the protocol. Results showed the pertinence of such modifications and revealed the usefulness and high quality of mentioned ink, which successfully stains all fungal structures allowing a clear visualization during long time. 2015 artículo científico 1819-4087 https://www.redalyc.org/articulo.oa?id=193239249003 es http://www.redalyc.org/revista.oa?id=1932 Cultivos Tropicales application/pdf Instituto Nacional de Ciencias Agrícolas Cultivos Tropicales (Cuba) Num.2 Vol.36 |
| title | ALTERNATIVA DE LA TÉCNICA DE TINCIÓN PARA DETERMINAR LA COLONIZACIÓN MICORRÍZICA |
| topic | Agrociencias roots colorants health protection Arbuscular mycorrhiza |
| url | https://www.redalyc.org/articulo.oa?id=193239249003 |