Initial intracellular proteome profile of Aspergillus niger biofilms
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| Format: | Artículo científico |
| Langue: | en |
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Universidad Nacional Mayor de San Marcos
2009
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| _version_ | 1876433865017393152 |
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| author | Gretty K. Villena |
| author_facet | Gretty K. Villena |
| contents | Initial intracellular proteome profile of Aspergillus niger biofilms Gretty K. Villena Lavanya Venkatesh Akihiro Yamazaki Shinji Tsuyumu Marcel Gutiérrez-Correa Biología MS 2D TOF PAGE biofilms An initial profiling of the intracellular proteome of Aspergillus niger ATCC 10864 biofilm cultures developed on polyester cloth was carried out by using 2D-PAGE and MS-TOF analysis and it was compared to the proteome of conventionally grown free-living submerged cultures. A number of 2D-PAGE protein spots from both types of cultures were subjected to MS-TOF analysis and data interrogation of the NCBI nr database available for this species. Proteomic maps showed different expression patterns in both culture systems with differentially expressed proteins in each case. In biofilm cultures, 19% and 32% of the selected protein spots were overexpressed and differentially expressed, respectively. On the contrary, in free-living cultures, 44% and 7% of the selected protein spots were over-expressed and differentially expressed, respectively. Although preliminary, results presented in this paper show that there are significant differences between the proteomes of A. niger biofilm and free-living mycelia. It seems that cell adhesion is the most important stimulus responsible for biofilm development which is the basis of Surface Adhesion Fermentation. 2009 artículo científico 1561-0837 https://www.redalyc.org/articulo.oa?id=195014940013 en http://www.redalyc.org/revista.oa?id=1950 Revista Peruana de Biología application/pdf Universidad Nacional Mayor de San Marcos Revista Peruana de Biología (Perú) Num.1 Vol.16 |
| format | Artículo científico |
| id | redalyc_195014940013 |
| institution | Redalyc |
| language | en |
| publishDate | 2009 |
| publisher | Universidad Nacional Mayor de San Marcos |
| spellingShingle | Initial intracellular proteome profile of Aspergillus niger biofilms Gretty K. Villena Biología MS 2D TOF PAGE biofilms Initial intracellular proteome profile of Aspergillus niger biofilms Gretty K. Villena Lavanya Venkatesh Akihiro Yamazaki Shinji Tsuyumu Marcel Gutiérrez-Correa Biología MS 2D TOF PAGE biofilms An initial profiling of the intracellular proteome of Aspergillus niger ATCC 10864 biofilm cultures developed on polyester cloth was carried out by using 2D-PAGE and MS-TOF analysis and it was compared to the proteome of conventionally grown free-living submerged cultures. A number of 2D-PAGE protein spots from both types of cultures were subjected to MS-TOF analysis and data interrogation of the NCBI nr database available for this species. Proteomic maps showed different expression patterns in both culture systems with differentially expressed proteins in each case. In biofilm cultures, 19% and 32% of the selected protein spots were overexpressed and differentially expressed, respectively. On the contrary, in free-living cultures, 44% and 7% of the selected protein spots were over-expressed and differentially expressed, respectively. Although preliminary, results presented in this paper show that there are significant differences between the proteomes of A. niger biofilm and free-living mycelia. It seems that cell adhesion is the most important stimulus responsible for biofilm development which is the basis of Surface Adhesion Fermentation. 2009 artículo científico 1561-0837 https://www.redalyc.org/articulo.oa?id=195014940013 en http://www.redalyc.org/revista.oa?id=1950 Revista Peruana de Biología application/pdf Universidad Nacional Mayor de San Marcos Revista Peruana de Biología (Perú) Num.1 Vol.16 |
| title | Initial intracellular proteome profile of Aspergillus niger biofilms |
| topic | Biología MS 2D TOF PAGE biofilms |
| url | https://www.redalyc.org/articulo.oa?id=195014940013 |