Effect of high-voltage electrical stimulation on the albumin and histamine serum concentrations, edema, and pain in acute joint inflammation of rats

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Main Author: Maria C. Sandoval
Format: Artículo científico
Language:en
Published: Associação Brasileira de Pesquisa e Pós-Graduação em Fisioterapia 2015
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author Maria C. Sandoval
author_facet Maria C. Sandoval
contents Effect of high-voltage electrical stimulation on the albumin and histamine serum concentrations, edema, and pain in acute joint inflammation of rats Maria C. Sandoval Carolina R. Ramirez Diana M. Camargo Thiago L. Russo Tania F. Salvini Salud sprain inflammation Physical therapy electrical stimulation Background: The mechanism by which high-voltage electrical stimulation (HVPC) acts on edema reduction is unknown. Objective: To assess the effect of HVPC with negative polarity (-) applied to the ankle of rats with acute joint inflammation. Method: Sixty-four rats were divided into four groups (n=16): inflamed+HVPC(-), 0.03 mL application of ι-carrageenan (3%) to the tibiotarsal joint plus HVPC(-); inflamed+HVPC placebo, carrageenan application and HVPC placebo; normal+HVPC(-), HVPC application(-); and normal control, no intervention. The HVPC(-) 100 Hz at a submotor level was applied daily for 45 min on three consecutive days. The variables were pain, hind-foot volume, and serum histamine and albumin assessed before and during the 48 hours following inflammation. The variables were compared using the t test, one-way ANOVA, nested ANOVA for repeated measures, and the post hoc Bonferroni test. Analysis of covariance was applied to adjust the effects of HVPC(-) by measurements of pain, inflammation, albumin, and histamine at 24 h, and the final weight was compared to the other groups. The significance level was set at p<0.05. Results: There were no differences between the inflamed+HVPC(-) and inflamed+HVPC placebo groups in terms of pain or edema (p>0.05). Albumin was reduced in the groups that received the intervention, but there was no differences between them. There was only a 24 hour increase in histamine with the normal+HVPC(-) (p=0.0001) and inflamed+HVPC placebo groups (p=0.01) compared to the normal control group. Conclusions: The results of the present study suggest that HVPC(-) with the parameters employed did not reduce pain or edema and did not change serum albumin or histamine levels,, which indicates the inability of this resource to have a positive effect when treating treat acute joint inflammation. 2015 artículo científico 1413-3555 https://www.redalyc.org/articulo.oa?id=235037745002 en http://www.redalyc.org/revista.oa?id=2350 Revista Brasileira de Fisioterapia application/pdf Associação Brasileira de Pesquisa e Pós-Graduação em Fisioterapia Revista Brasileira de Fisioterapia (Brasil) Num.2 Vol.19
format Artículo científico
id redalyc_235037745002
institution Redalyc
language en
publishDate 2015
publisher Associação Brasileira de Pesquisa e Pós-Graduação em Fisioterapia
spellingShingle Effect of high-voltage electrical stimulation on the albumin and histamine serum concentrations, edema, and pain in acute joint inflammation of rats
Maria C. Sandoval
Salud
sprain
inflammation
Physical therapy
electrical stimulation
Effect of high-voltage electrical stimulation on the albumin and histamine serum concentrations, edema, and pain in acute joint inflammation of rats Maria C. Sandoval Carolina R. Ramirez Diana M. Camargo Thiago L. Russo Tania F. Salvini Salud sprain inflammation Physical therapy electrical stimulation Background: The mechanism by which high-voltage electrical stimulation (HVPC) acts on edema reduction is unknown. Objective: To assess the effect of HVPC with negative polarity (-) applied to the ankle of rats with acute joint inflammation. Method: Sixty-four rats were divided into four groups (n=16): inflamed+HVPC(-), 0.03 mL application of ι-carrageenan (3%) to the tibiotarsal joint plus HVPC(-); inflamed+HVPC placebo, carrageenan application and HVPC placebo; normal+HVPC(-), HVPC application(-); and normal control, no intervention. The HVPC(-) 100 Hz at a submotor level was applied daily for 45 min on three consecutive days. The variables were pain, hind-foot volume, and serum histamine and albumin assessed before and during the 48 hours following inflammation. The variables were compared using the t test, one-way ANOVA, nested ANOVA for repeated measures, and the post hoc Bonferroni test. Analysis of covariance was applied to adjust the effects of HVPC(-) by measurements of pain, inflammation, albumin, and histamine at 24 h, and the final weight was compared to the other groups. The significance level was set at p<0.05. Results: There were no differences between the inflamed+HVPC(-) and inflamed+HVPC placebo groups in terms of pain or edema (p>0.05). Albumin was reduced in the groups that received the intervention, but there was no differences between them. There was only a 24 hour increase in histamine with the normal+HVPC(-) (p=0.0001) and inflamed+HVPC placebo groups (p=0.01) compared to the normal control group. Conclusions: The results of the present study suggest that HVPC(-) with the parameters employed did not reduce pain or edema and did not change serum albumin or histamine levels,, which indicates the inability of this resource to have a positive effect when treating treat acute joint inflammation. 2015 artículo científico 1413-3555 https://www.redalyc.org/articulo.oa?id=235037745002 en http://www.redalyc.org/revista.oa?id=2350 Revista Brasileira de Fisioterapia application/pdf Associação Brasileira de Pesquisa e Pós-Graduação em Fisioterapia Revista Brasileira de Fisioterapia (Brasil) Num.2 Vol.19
title Effect of high-voltage electrical stimulation on the albumin and histamine serum concentrations, edema, and pain in acute joint inflammation of rats
topic Salud
sprain
inflammation
Physical therapy
electrical stimulation
url https://www.redalyc.org/articulo.oa?id=235037745002