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| Format: | Artículo científico |
| Language: | en |
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Universidade Federal de Goiás
2011
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| Online Access: | https://www.redalyc.org/articulo.oa?id=253019709018 |
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Table of Contents:
- GROWTH RATE OF MIXED RUMINAL BACTERIA AS A FUNCTION OF ENERGETIC SUBSTRATE CONCENTRATION IN BATH CULTURE Tadeu Silva de Oliveira Rogério de Paula Lana José Carlos Pereira Geicimara Guimarães José Alípio Faleiro Neto Agrociencias pH Burk Menten sucrose Michaelis The objective of this study was to evaluate levels of sucrose on microbial growth. Roll tubes were filled with 15.6 mL of buffer Chen, 1 mL of inocula (rumen fluid from fistulated steer at pasture), 1 mL of Trypticase or hydrolyzed casein (1 g L-1 as final concentration) and 2.4 mL of sucrose solutions (0.00 g L-1, 0.37 g L-1, 0.75 g L-1, 1.5 g L-1, 3.0 g L-1, 6.0 g L-1, 12.0 g L-1, and 24.0 g L-1), in triplicate. The pH was 5.56 at 0 h and 4.6 after 6 h, and 5.83 in 0 g L-1 and 4.18 above 1.5 g L-1 of sucrose. The optical density (OD-600 nm) and microbial protein (MP-595 nm) tended to stabilize after 12 h and 6 g L-1 of sucrose, with no interaction between them. The OD (6 h to 24 h mean values) was curvilinear (hyperbolic), as a function of sucrose concentration, followed the saturation kinetics of Michaelis-Menten, which is typical of enzymes systems, and was described by the following Lineweaver-Burk equation: 1/OD = 0.508*(1/sucrose) + 1.15, R² = 0.99. The theoretical maximum microbial growth (1/a = kmax) was 0.87 OD and the concentration of sucrose to reach half of kmax (b/a = Ks) was 0.44 g L-1 of sucrose. The microbial growth was affected by incubation time, substrate concentration, and culture medium acidification. 2011 artículo científico 1517-6398 https://www.redalyc.org/articulo.oa?id=253019709018 en http://www.redalyc.org/revista.oa?id=2530 Pesquisa Agropecuária Tropical application/pdf Universidade Federal de Goiás Pesquisa Agropecuária Tropical (Brasil) Num.1 Vol.41