Leishmania (Viannia) panamensis expresses a nuclease with molecular and biochemical features similar to the Endonuclease G of higher eukaryotes
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| Format: | Artículo científico |
| Language: | en |
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Universidad del Valle
2011
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| author | MIGUEL A. TORO-LONDOÑO |
| author_facet | MIGUEL A. TORO-LONDOÑO |
| contents | Leishmania (Viannia) panamensis expresses a nuclease with molecular and biochemical features similar to the Endonuclease G of higher eukaryotes MIGUEL A. TORO-LONDOÑO EDWIN BAIRON PATIÑO SARA MARÍA ROBLEDO ANTONIO JIMÉNEZ-RUIZ JUAN FERNANDO ALZATE Medicina Refolding Phylogeny Endonuclease G Recombinant proteins Leishmania panamensis Objective: To characterize the molecular and biochemical features of the Endonuclease G of Leishmania (Viannia) panamensis. Methods: The gene of the putative L. (V.) panamensis Endonuclease G was amplified, cloned, and sequenced. The recombinant protein was produced in a heterologous expression system and biochemical assays were run to determine its ion, temperature, and pH preferences. Results: The L. (V.) panamensis rENDOG has biochemical features similar to those found in other trypanosomatids and higher eukaryotes. In addition, phylogenetic analysis revealed a possible evolutionary relationship with metazoan ENDOG. Conclusions: L. (V.) panamensis has a gene that codifies an ENDOG homologous to those of higher organisms. This enzyme can be produced in Escherichia coli and is able to degrade covalently closed circular double-stranded DNA. It has a magnesium preference, can be inhibited by potassium, and is able to function within a wide temperature and pH range. 2011 artículo científico 0120-8322 https://www.redalyc.org/articulo.oa?id=28318450004 en http://www.redalyc.org/revista.oa?id=283 Colombia Médica application/pdf Universidad del Valle Colombia Médica (Colombia) Num.2 Vol.42 |
| format | Artículo científico |
| id | redalyc_28318450004 |
| institution | Redalyc |
| language | en |
| publishDate | 2011 |
| publisher | Universidad del Valle |
| spellingShingle | Leishmania (Viannia) panamensis expresses a nuclease with molecular and biochemical features similar to the Endonuclease G of higher eukaryotes MIGUEL A. TORO-LONDOÑO Medicina Refolding Phylogeny Endonuclease G Recombinant proteins Leishmania panamensis Leishmania (Viannia) panamensis expresses a nuclease with molecular and biochemical features similar to the Endonuclease G of higher eukaryotes MIGUEL A. TORO-LONDOÑO EDWIN BAIRON PATIÑO SARA MARÍA ROBLEDO ANTONIO JIMÉNEZ-RUIZ JUAN FERNANDO ALZATE Medicina Refolding Phylogeny Endonuclease G Recombinant proteins Leishmania panamensis Objective: To characterize the molecular and biochemical features of the Endonuclease G of Leishmania (Viannia) panamensis. Methods: The gene of the putative L. (V.) panamensis Endonuclease G was amplified, cloned, and sequenced. The recombinant protein was produced in a heterologous expression system and biochemical assays were run to determine its ion, temperature, and pH preferences. Results: The L. (V.) panamensis rENDOG has biochemical features similar to those found in other trypanosomatids and higher eukaryotes. In addition, phylogenetic analysis revealed a possible evolutionary relationship with metazoan ENDOG. Conclusions: L. (V.) panamensis has a gene that codifies an ENDOG homologous to those of higher organisms. This enzyme can be produced in Escherichia coli and is able to degrade covalently closed circular double-stranded DNA. It has a magnesium preference, can be inhibited by potassium, and is able to function within a wide temperature and pH range. 2011 artículo científico 0120-8322 https://www.redalyc.org/articulo.oa?id=28318450004 en http://www.redalyc.org/revista.oa?id=283 Colombia Médica application/pdf Universidad del Valle Colombia Médica (Colombia) Num.2 Vol.42 |
| title | Leishmania (Viannia) panamensis expresses a nuclease with molecular and biochemical features similar to the Endonuclease G of higher eukaryotes |
| topic | Medicina Refolding Phylogeny Endonuclease G Recombinant proteins Leishmania panamensis |
| url | https://www.redalyc.org/articulo.oa?id=28318450004 |