Rapid detection of sentinel lymph node metastases in different techniques and comparison in low-grade breast carcinomas
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Universidad del Valle
2012
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| author | Luz F. Sua |
| author_facet | Luz F. Sua |
| contents | Rapid detection of sentinel lymph node metastases in different techniques and comparison in low-grade breast carcinomas Luz F. Sua Nhora M. Silva Martha Vidaurreta Virginia de la Orden Silvia Veganzones Sara Rafael María L. Maestro de las Casas Medicina Biopsy Breast neoplasm Lymph node excision Lymphatic metastasis Immunohistochemistry Introduction: The role of sentinel node biopsy has revolutionized breast cancer treatment. This determination reduces the mobility of a complete axillary lymphadenectomy. The aim of our study is to analyze the value of sentinel node in low-grade histological breast tumors, studied with hematoxylin and eosin techniques, Immunohistochemistry, and molecular chain reaction in real-time quantitative polymerase (RT-PCR). Materials and methods: In a pilot study we studied a total of 21 patients with histologicaldiagnosis of mucinous carcinoma, adenoid cystic carcinoma, and medullar carcinoma that underwent the sentinel node technique. Once thelymph node was removed, it was sent to pathology, where it was fragmented and evaluated,using between 25% and 50% of the lymph node for molecular biology laboratory studies. Results: The sentinel nodes studied were 32, corresponding to the 21 patients. Of the 32 lymphnodes analyzed, 29 (90.6%) were negative on histopathological examination and the molecular identification, 2 (6.2%) were positive in both techniques and 1 (3.125%) lymph node was positive with quantitative RT-PCR and negative in histology(H&E), which - subsequently by immunohistochemistry (IHC) - was diagnosed as isolated tumor cells (ITC). Conclusion: When comparing the techniques of hematoxylin and eosin, immunohistochemistry, and molecular RT-PCR technique, we found greater sensitivity of molecular techniques; this can reduce the false negative and improve diagnosis of sentinel node metastases. Patients with low histological grade carcinomashave high survival rates, less aggressive tumor behavior, and reduced lymph node at diagnosis. 2012 artículo científico 0120-8322 https://www.redalyc.org/articulo.oa?id=28323202004 en http://www.redalyc.org/revista.oa?id=283 Colombia Médica application/pdf Universidad del Valle Colombia Médica (Colombia) Num.1 Vol.43 |
| format | Artículo científico |
| id | redalyc_28323202004 |
| institution | Redalyc |
| language | en |
| publishDate | 2012 |
| publisher | Universidad del Valle |
| spellingShingle | Rapid detection of sentinel lymph node metastases in different techniques and comparison in low-grade breast carcinomas Luz F. Sua Medicina Biopsy Breast neoplasm Lymph node excision Lymphatic metastasis Immunohistochemistry Rapid detection of sentinel lymph node metastases in different techniques and comparison in low-grade breast carcinomas Luz F. Sua Nhora M. Silva Martha Vidaurreta Virginia de la Orden Silvia Veganzones Sara Rafael María L. Maestro de las Casas Medicina Biopsy Breast neoplasm Lymph node excision Lymphatic metastasis Immunohistochemistry Introduction: The role of sentinel node biopsy has revolutionized breast cancer treatment. This determination reduces the mobility of a complete axillary lymphadenectomy. The aim of our study is to analyze the value of sentinel node in low-grade histological breast tumors, studied with hematoxylin and eosin techniques, Immunohistochemistry, and molecular chain reaction in real-time quantitative polymerase (RT-PCR). Materials and methods: In a pilot study we studied a total of 21 patients with histologicaldiagnosis of mucinous carcinoma, adenoid cystic carcinoma, and medullar carcinoma that underwent the sentinel node technique. Once thelymph node was removed, it was sent to pathology, where it was fragmented and evaluated,using between 25% and 50% of the lymph node for molecular biology laboratory studies. Results: The sentinel nodes studied were 32, corresponding to the 21 patients. Of the 32 lymphnodes analyzed, 29 (90.6%) were negative on histopathological examination and the molecular identification, 2 (6.2%) were positive in both techniques and 1 (3.125%) lymph node was positive with quantitative RT-PCR and negative in histology(H&E), which - subsequently by immunohistochemistry (IHC) - was diagnosed as isolated tumor cells (ITC). Conclusion: When comparing the techniques of hematoxylin and eosin, immunohistochemistry, and molecular RT-PCR technique, we found greater sensitivity of molecular techniques; this can reduce the false negative and improve diagnosis of sentinel node metastases. Patients with low histological grade carcinomashave high survival rates, less aggressive tumor behavior, and reduced lymph node at diagnosis. 2012 artículo científico 0120-8322 https://www.redalyc.org/articulo.oa?id=28323202004 en http://www.redalyc.org/revista.oa?id=283 Colombia Médica application/pdf Universidad del Valle Colombia Médica (Colombia) Num.1 Vol.43 |
| title | Rapid detection of sentinel lymph node metastases in different techniques and comparison in low-grade breast carcinomas |
| topic | Medicina Biopsy Breast neoplasm Lymph node excision Lymphatic metastasis Immunohistochemistry |
| url | https://www.redalyc.org/articulo.oa?id=28323202004 |