Gespeichert in:
| 1. Verfasser: | |
|---|---|
| Format: | Artículo científico |
| Sprache: | en |
| Veröffentlicht: |
Universidad del Valle
2012
|
| Schlagworte: | |
| Online-Zugang: | https://www.redalyc.org/articulo.oa?id=28323202005 |
| Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
| _version_ | 1866556695702929408 |
|---|---|
| author | María Consuelo Vanegas |
| author_facet | María Consuelo Vanegas |
| contents | Molecular serotyping and identification of the 85M gragment in different Colombian isolates of Listeria monocytogenes strains: A descriptive study María Consuelo Vanegas Mayra Viviana Medrano Aida Juliana Martínez Stefany Alejandra Arévalo Medicina Food Serotypes Isolation Listeriosis 85M fragment Introduction: Listeria monocytogenes is a pathogen acquired through the consumption of contaminated foods.Thirteen serotypes have been reported, of which 1/2a, 1/2b, and 4b are responsible for 98% of human listeriosis cases. This study examines the association between serotypes and virulent clones, offering greaterinformation and providing tools to prevent and control diseases caused by L. monocytogenes serotype 4b.Objective: To identify the serotypes from L. monocytogene strains isolated from different samples by performingthe molecular subtyping technique; to determine the 85M fragment that codifies for epidemic cloneI. Methods : 108 strains of L. monocytogenes were used, isolated from samples of animals, bodyfluids, foods, and food processing plant equipment and spaces. The samples were identified by following the Bacteriological AnalyticalManual protocol described by the Food and Drug Administration (FDA). The strains were identifiedby Polymerase Chain Reaction (PCR) using primers and a standardized protocol from a previous research project. Serotypeidentification was performed by multiplex PCR. The determination of the 85M fragment of the SSCS cassette was done by following the protocol by Yildrim et al. Results : Of the 108 L. monocytogenes strains analyzed, 60.2% (65 strains) belonged to the 4b-4d-4e serotype, 17.6% (19 strains) were identified as 1/2a-3a serotype, 14.8% (16 strains) were 4a-4c serotype, 3.7%(4 strains) belonged tothe 1/2c-3c serotype, and (3.7%) corresponded to the 1/2b-3b-7 serotype. It was determined thatthe L. monocytogenes strains serotype 4b-4d-4e and 1/2a-3b have the 85M fragment of the SSCS cassette. Conclusion : This study reports the predominant existence of L. monocytogenes strains serotype 4b-4d-4e in food, environmental, and clinical samples. The presence of an epidemic clone I region was also found in L. monocytogenes strains. 2012 artículo científico 0120-8322 https://www.redalyc.org/articulo.oa?id=28323202005 en http://www.redalyc.org/revista.oa?id=283 Colombia Médica application/pdf Universidad del Valle Colombia Médica (Colombia) Num.1 Vol.43 |
| format | Artículo científico |
| id | redalyc_28323202005 |
| language | en |
| publishDate | 2012 |
| publisher | Universidad del Valle |
| spellingShingle | Molecular serotyping and identification of the 85M gragment in different Colombian isolates of Listeria monocytogenes strains: A descriptive study María Consuelo Vanegas Medicina Food Serotypes Isolation Listeriosis 85M fragment Molecular serotyping and identification of the 85M gragment in different Colombian isolates of Listeria monocytogenes strains: A descriptive study María Consuelo Vanegas Mayra Viviana Medrano Aida Juliana Martínez Stefany Alejandra Arévalo Medicina Food Serotypes Isolation Listeriosis 85M fragment Introduction: Listeria monocytogenes is a pathogen acquired through the consumption of contaminated foods.Thirteen serotypes have been reported, of which 1/2a, 1/2b, and 4b are responsible for 98% of human listeriosis cases. This study examines the association between serotypes and virulent clones, offering greaterinformation and providing tools to prevent and control diseases caused by L. monocytogenes serotype 4b.Objective: To identify the serotypes from L. monocytogene strains isolated from different samples by performingthe molecular subtyping technique; to determine the 85M fragment that codifies for epidemic cloneI. Methods : 108 strains of L. monocytogenes were used, isolated from samples of animals, bodyfluids, foods, and food processing plant equipment and spaces. The samples were identified by following the Bacteriological AnalyticalManual protocol described by the Food and Drug Administration (FDA). The strains were identifiedby Polymerase Chain Reaction (PCR) using primers and a standardized protocol from a previous research project. Serotypeidentification was performed by multiplex PCR. The determination of the 85M fragment of the SSCS cassette was done by following the protocol by Yildrim et al. Results : Of the 108 L. monocytogenes strains analyzed, 60.2% (65 strains) belonged to the 4b-4d-4e serotype, 17.6% (19 strains) were identified as 1/2a-3a serotype, 14.8% (16 strains) were 4a-4c serotype, 3.7%(4 strains) belonged tothe 1/2c-3c serotype, and (3.7%) corresponded to the 1/2b-3b-7 serotype. It was determined thatthe L. monocytogenes strains serotype 4b-4d-4e and 1/2a-3b have the 85M fragment of the SSCS cassette. Conclusion : This study reports the predominant existence of L. monocytogenes strains serotype 4b-4d-4e in food, environmental, and clinical samples. The presence of an epidemic clone I region was also found in L. monocytogenes strains. 2012 artículo científico 0120-8322 https://www.redalyc.org/articulo.oa?id=28323202005 en http://www.redalyc.org/revista.oa?id=283 Colombia Médica application/pdf Universidad del Valle Colombia Médica (Colombia) Num.1 Vol.43 |
| title | Molecular serotyping and identification of the 85M gragment in different Colombian isolates of Listeria monocytogenes strains: A descriptive study |
| topic | Medicina Food Serotypes Isolation Listeriosis 85M fragment |
| url | https://www.redalyc.org/articulo.oa?id=28323202005 |