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Main Author:	Salim Máttar
Format:	Artículo científico
Language:	es
Published:	Universidad del Valle 2005
Subjects:	Medicina Colombia Flavivirus Virus del Nilo Seroreacciones Serodiagnóstico
Online Access:	https://www.redalyc.org/articulo.oa?id=28336308
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Limitaciones para el serodiagnóstico del virus del oeste del Nilo en zonas endémicas con co-circulación de Flavivirus en el Caribe colombiano Salim Máttar Miguel Parra José Torres Medicina Colombia Flavivirus Virus del Nilo Seroreacciones Serodiagnóstico Introducción: Esta es la primera aproximación sero-epidemiológica que se hace en Colombia sobre el virus del Oeste del Nilo(VON).Objetivo: El propósito de este estudio fue el de establecer la presencia de anticuerpos IgG contra el VON en una población dela costa atlántica utilizando dos pruebas comerciales y establecer su utilidad.Materiales y método: Se analizaron de forma aleatoria 52 muestras de sueros de personas que trabajaban en labores agrícolasdesde hacia más de 15 años en el departamento de Sucre. Se utilizó la técnica West Nile Virus IgG ELISA (Focus Technologies).También se utilizó la prueba de inmunofluorescencia (PANBIO Columbia. Arbovirus IgG-IFA slides), donde se evaluó la detecciónde anticuerpos contra cinco antígenos diferentes de arbovirus (encefalitis equina venezolana, encefalitis japonesa, fiebre amarillay dengue).Resultados: Con la prueba de ELISA, de 52 sueros estudiados para la detección de anticuerpos contra VON, 38 (73%) resultaronpositivos, los 14 (27%) restantes resultaron negativos. Con la prueba de IFA, 6 sueros (11.5%) resultaron seropositivos débilespara VON, 46 (88.5%) resultaron negativos para la detección de anticuerpos contra el VON. Utilizando IFA se presentaron reaccionescruzadas contra otros arbovirus como dengue, encefalitis venezolana y japonesa y fiebre amarilla. El estudio demostró lacomplejidad del serodiagnóstico de Flavivirus en las zonas endémicas como la del Caribe colombiano.Conclusión: Los resultados obtenidos en este estudio demuestran que las pruebas de ELISA e IFA para humanos son de pocautilidad diagnóstica contra el VON en las zonas colombianas donde se presentan circulación de otros Flavivirus como dengue,fiebre amarilla o encefalitis equina. 2005 artículo científico 0120-8322 https://www.redalyc.org/articulo.oa?id=28336308 es http://www.redalyc.org/revista.oa?id=283 Colombia Médica application/pdf Universidad del Valle Colombia Médica (Colombia) Num.3 Vol.36

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// Anecdotal testing Today and Yesterday seems to indicate destroy() // is called by the garbage collector and everything is good. // Something to keep in mind though. return 0; } /** * Write function that is called when session data is to be saved. * * @param string $sessId The current session ID * @param string $data The session data to write * * @return bool */ public function write($sessId, $data): bool { if ($this->writesDisabled) { return true; } return $this->saveSession($sessId, $data); } /** * A function that is called internally when session data is to be saved. * * @param string $sessId The current session ID * @param string $data The session data to write * * @return bool */ abstract protected function saveSession($sessId, $data): bool; }

* * @return void */ public function writeClose() { // The assumption is that we're using PHP's ext/session. // session_write_close() will actually overwrite $_SESSION with an // empty array on completion -- which leads to a mismatch between what // is in the storage object and $_SESSION. To get around this, we // temporarily reset $_SESSION to an array, and then re-link it to // the storage object. // // Additionally, while you _can_ write to $_SESSION following a // session_write_close() operation, no changes made to it will be // flushed to the session handler. As such, we now mark the storage // object isImmutable. $storage = $this->getStorage(); if (! $storage->isImmutable()) { $_SESSION = $storage->toArray(true); session_write_close(); $storage->fromArray($_SESSION); $storage->markImmutable(); } } /** * Attempt to set the session name * * If the session has already been started, or if the name provided fails * validation, an exception will be raised. * * @param string $name * @return SessionManager * @throws Exception\InvalidArgumentException */ public function setName($name) { if ($this->sessionExists()) { throw new Exception\InvalidArgumentException( 'Cannot set session name after a session has already started'

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