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| Format: | Artículo científico |
| Language: | en |
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Universidad del Valle
2007
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| Online Access: | https://www.redalyc.org/articulo.oa?id=28338106 |
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| _version_ | 1866817035756896256 |
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| author | Jorge Hernán Ramírez |
| author_facet | Jorge Hernán Ramírez |
| contents | Implementation of the technique in isolated organ vascular as tool for the validation of medicinal plants: Study of the vasodilator effect of the S. scutellarioides Jorge Hernán Ramírez Mauricio Palacios Oscar Gutiérrez Medicina Salvia Mastranto Hypertension Vasoconstriction Ethnopharmacology Introduction: Salvia scutellarioides is a plant with antihypertensive and diuretic properties. In this study, its vasodilator effectis evaluated using the isolated organ technique.Objectives: To standardize the isolated vascular organ, and to study the effect of S. scutellarioides in vasoconstriction by a1receptors stimulation.Methodology: Rat aortic rings were placed in an organ bath equipment (BIOPAC®), at 37o C temperature and constant carbogengas bubbling. Tension changes were registered with an isometric tension transducer and data acquisition equipment. During thestandardization, six curves of concentration-tension with phenylephrine at 1, 3 and 5 hours were registered, in order to determinemaximum tension (TM), and the effective concentration 50 (EC50). Finally, three concentration-tension curves were obtained forphenylephrine with S. scutellarioides at a concentration of 10 mg/dl and 20 mg/dl.Results: During the technique standardization, no statistically significant differences were found in TM for phenylephrine at 1,3 and 5 hours. The administration of S. scutellarioides produced a TM dose dependent reduction with no EC50 changes.Discussion: The implemented isolated organ technique is viable up to 5 hours, and does not produce a1 receptorsdesensibilization. The TM diminution by S. scutellarioides is explained by a non competitive a1 antagonism effect. Studies to isolateactive principles for allowing the initiation of phase I human clinical trials are required 2007 artículo científico 0120-8322 https://www.redalyc.org/articulo.oa?id=28338106 en http://www.redalyc.org/revista.oa?id=283 Colombia Médica application/pdf Universidad del Valle Colombia Médica (Colombia) Num.1 Vol.38 |
| format | Artículo científico |
| id | redalyc_28338106 |
| language | en |
| publishDate | 2007 |
| publisher | Universidad del Valle |
| spellingShingle | Implementation of the technique in isolated organ vascular as tool for the validation of medicinal plants: Study of the vasodilator effect of the S. scutellarioides Jorge Hernán Ramírez Medicina Salvia Mastranto Hypertension Vasoconstriction Ethnopharmacology Implementation of the technique in isolated organ vascular as tool for the validation of medicinal plants: Study of the vasodilator effect of the S. scutellarioides Jorge Hernán Ramírez Mauricio Palacios Oscar Gutiérrez Medicina Salvia Mastranto Hypertension Vasoconstriction Ethnopharmacology Introduction: Salvia scutellarioides is a plant with antihypertensive and diuretic properties. In this study, its vasodilator effectis evaluated using the isolated organ technique.Objectives: To standardize the isolated vascular organ, and to study the effect of S. scutellarioides in vasoconstriction by a1receptors stimulation.Methodology: Rat aortic rings were placed in an organ bath equipment (BIOPAC®), at 37o C temperature and constant carbogengas bubbling. Tension changes were registered with an isometric tension transducer and data acquisition equipment. During thestandardization, six curves of concentration-tension with phenylephrine at 1, 3 and 5 hours were registered, in order to determinemaximum tension (TM), and the effective concentration 50 (EC50). Finally, three concentration-tension curves were obtained forphenylephrine with S. scutellarioides at a concentration of 10 mg/dl and 20 mg/dl.Results: During the technique standardization, no statistically significant differences were found in TM for phenylephrine at 1,3 and 5 hours. The administration of S. scutellarioides produced a TM dose dependent reduction with no EC50 changes.Discussion: The implemented isolated organ technique is viable up to 5 hours, and does not produce a1 receptorsdesensibilization. The TM diminution by S. scutellarioides is explained by a non competitive a1 antagonism effect. Studies to isolateactive principles for allowing the initiation of phase I human clinical trials are required 2007 artículo científico 0120-8322 https://www.redalyc.org/articulo.oa?id=28338106 en http://www.redalyc.org/revista.oa?id=283 Colombia Médica application/pdf Universidad del Valle Colombia Médica (Colombia) Num.1 Vol.38 |
| title | Implementation of the technique in isolated organ vascular as tool for the validation of medicinal plants: Study of the vasodilator effect of the S. scutellarioides |
| topic | Medicina Salvia Mastranto Hypertension Vasoconstriction Ethnopharmacology |
| url | https://www.redalyc.org/articulo.oa?id=28338106 |