Microarray analysis of the in vitro granulomatous response to Mycobacterium tuberculosis H37Ra
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Universidad del Valle
2015
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| _version_ | 1876425066898522112 |
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| author | Niradiz Reyes |
| author_facet | Niradiz Reyes |
| contents | Microarray analysis of the in vitro granulomatous response to Mycobacterium tuberculosis H37Ra Niradiz Reyes Alfonso Bettin Ismael Reyes Jan Geliebter Medicina granuloma chemokines Mycobacterium tuberculosis oligonucleotide microarrays Background: The hallmark of tuberculosis is the granuloma, an organized cellular accumulation playing a key role in host defense against Mycobacterium tuberculosis. These structures sequester and contain mycobacterial cells preventing active disease, while long term maintenance of granulomas leads to latent disease. Clear understanding on mechanisms involved in granuloma formation and maintenance is lacking. Objective: to monitor granuloma formation and to determine gene expression profiles induced during the granulomatous response to M. tuberculosis (H37Ra). Methods: We used a previously characterized in vitro human model. Cellular aggregation was followed daily with microscopy and Wright staining for 5 days. Granulomas were collected at 24h, RNA extracted and hybridized to Affymetrix human microarrays. Results: Daily microscopic examination revealed gradual formation of granulomas in response to mycobacterial infection. Granulomatous structures persisted for 96 h, and then began to disappear. Conclusions: Microarray analysis identified genes in the innate immune response and antigen presentation pathways activated during the in vitro granulomatous response to live mycobacterial cells, revealing very early changes in gene expression of the human granulomatous response. 2015 artículo científico 0120-8322 https://www.redalyc.org/articulo.oa?id=28338620005 https://www.redalyc.org/journal/283/28338620005/ https://www.redalyc.org/journal/283/28338620005/html/ https://www.redalyc.org/journal/283/28338620005/28338620005.epub https://www.redalyc.org/journal/283/28338620005/movil en http://www.redalyc.org/revista.oa?id=283 Colombia Médica application/pdf Universidad del Valle Colombia Médica (Colombia) Num.1 Vol.46 |
| format | Artículo científico |
| id | redalyc_28338620005 |
| institution | Redalyc |
| language | en |
| publishDate | 2015 |
| publisher | Universidad del Valle |
| spellingShingle | Microarray analysis of the in vitro granulomatous response to Mycobacterium tuberculosis H37Ra Niradiz Reyes Medicina granuloma chemokines Mycobacterium tuberculosis oligonucleotide microarrays Microarray analysis of the in vitro granulomatous response to Mycobacterium tuberculosis H37Ra Niradiz Reyes Alfonso Bettin Ismael Reyes Jan Geliebter Medicina granuloma chemokines Mycobacterium tuberculosis oligonucleotide microarrays Background: The hallmark of tuberculosis is the granuloma, an organized cellular accumulation playing a key role in host defense against Mycobacterium tuberculosis. These structures sequester and contain mycobacterial cells preventing active disease, while long term maintenance of granulomas leads to latent disease. Clear understanding on mechanisms involved in granuloma formation and maintenance is lacking. Objective: to monitor granuloma formation and to determine gene expression profiles induced during the granulomatous response to M. tuberculosis (H37Ra). Methods: We used a previously characterized in vitro human model. Cellular aggregation was followed daily with microscopy and Wright staining for 5 days. Granulomas were collected at 24h, RNA extracted and hybridized to Affymetrix human microarrays. Results: Daily microscopic examination revealed gradual formation of granulomas in response to mycobacterial infection. Granulomatous structures persisted for 96 h, and then began to disappear. Conclusions: Microarray analysis identified genes in the innate immune response and antigen presentation pathways activated during the in vitro granulomatous response to live mycobacterial cells, revealing very early changes in gene expression of the human granulomatous response. 2015 artículo científico 0120-8322 https://www.redalyc.org/articulo.oa?id=28338620005 https://www.redalyc.org/journal/283/28338620005/ https://www.redalyc.org/journal/283/28338620005/html/ https://www.redalyc.org/journal/283/28338620005/28338620005.epub https://www.redalyc.org/journal/283/28338620005/movil en http://www.redalyc.org/revista.oa?id=283 Colombia Médica application/pdf Universidad del Valle Colombia Médica (Colombia) Num.1 Vol.46 |
| title | Microarray analysis of the in vitro granulomatous response to Mycobacterium tuberculosis H37Ra |
| topic | Medicina granuloma chemokines Mycobacterium tuberculosis oligonucleotide microarrays |
| url | https://www.redalyc.org/articulo.oa?id=28338620005 https://www.redalyc.org/journal/283/28338620005/ https://www.redalyc.org/journal/283/28338620005/html/ https://www.redalyc.org/journal/283/28338620005/28338620005.epub https://www.redalyc.org/journal/283/28338620005/movil |